We offer a variety of activated supports and accessories for the immobilization of proteins, antibodies, and other molecules. These resins are available separately or in convenient kits. Choose resins based on NHS-ester, AminoLink™, SulfoLink™, GlycoLink™, CarboxyLink™, and other activation technologies.
- Product choice—a variety of coupling chemistries to meet different immobilization strategies
- High performance—resins that maximize coupling efficiency and binding capacity
- Flexible—available in multiple pack sizes and formats
Choose the right immobilization solution for your experiment
|Pierce NHS-Activated Agarose||AminoLink Coupling Resin||AminoLink Plus Coupling Resin||SulfoLink Coupling Resin||GlycoLink Coupling Resin||CarboxyLink Coupling|
|Support||6% agarose (slurry or dry powder)||4% agarose||4% agarose, highly crosslinked||6% agarose||Ultralink (polyacrylamide/
|Coupling capacity||>25 mg/mL resin||1–20 mg/mL resin||20 mg/mL resin||20 mg/mL resin||1–10 mg/mL resin||5 mg/mL resin|
|30 min||4 hr||4 hr||2–3.5 hr||30 min||4 hr|
|Recommended for||Proteins||Proteins, antibodies||Proteins, antibodies||Proteins, peptides, antibodies||Glycoproteins, polyclonal antibodies||Unmodified peptides|
|Advantages||High coupling efficiency and capacity||High coupling efficiency||High coupling efficiency||Allows for gentle elution||Correctly orients antibodies during coupling||Flexible and gentle coupling conditions|
|Disadvantages||Narrow pH range (7–9)||May be coupled at antigen-binding site||May be coupled at antigen-binding site||Protein must be reduced first||Not recommended for monoclonal antibodies||Not recommended for antibodies|
|26196, 26197 (dry resin),
|20381 (10 mL), 20382 (50 mL)||20501 (10 mL), 20505 (50 mL)||20401 (10 mL), 20402 (50 mL), 20404 (250 mL)||53149 (10 mL)||20266 (25 mL)|
High-yield protein coupling
Immobilization efficiencies for various proteins on 1 mL of AminoLink Plus Resin when immobilized using the pH 7.2 coupling procedure.
|Protein||Protein (mg) reacted||Protein (mg) coupled||Coupling efficiency (%)|
*GAM-IgG is goat anti-mouse IgG, a secondary antibody.
Additional activated supports and accessories are available, as well as convenient kit formats that included disposable columns, buffers, and coupling reagents. Some activated supports are also available in magnetic bead formats.