Nature Protocols: Highly Efficient Neural Crest Differentiation
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This recent paper1 published in Nature Protocols by the laboratory of Stephen Dalton at the University of Georgia describes how to reprogram patient fibroblasts to induced pluripotent stem cells (iPSCs) using the CytoTune-iPS Reprogramming Kit, and then differentiate the iPSCs to neural crest stem cells in about 15 days using fully defined, feeder-free conditions.
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1Menendez L, Kulik MJ, Page AT et al. (2013) Directed differentiation of human pluripotent cells to neural crest stem cells. Nat Protoc Jan;8(1):203-12. Epub 2013 Jan 3.