Cotransfection refers to the simultaneous transfection with two separate nucleic acid molecules, such as plasmid DNA and siRNA. Cotransfection is a common procedure for stable transfection. The plasmid DNA may contain a gene that is easily assayed and acts as a marker.
For this to occur, a marker gene is co-transfected, which gives the cell a selection advantage, for example resistance towards a certain toxin. Selecting the right reagent for cotransfection is essential.
Featured cotransfection reagents
Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable superior transfection performance and reproducible results. It delivers exceptional transfection efficiency into the widest range of difficult-to-transfect and common cell types with improved cell viability.
- Learn more about Lipofectamine 3000
- Learn more about Lipofectamine 2000
Cotransfection Protocol with Lipofectamine 2000
- View the Cotransfection protocol