Cotransfection refers to the simultaneous transfection with two separate nucleic acid molecules, such as plasmid DNA and siRNA. Cotransfection is a common procedure for stable transfection. The plasmid DNA may contain a gene that is easily assayed and acts as a marker.

For this to occur, a marker gene is co-transfected, which gives the cell a selection  advantage, for example resistance towards a certain toxin. Selecting the right reagent for cotransfection is essential.

Featured cotransfection reagents

Lipofectamine 3000

Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable superior transfection performance and reproducible results. It delivers exceptional transfection efficiency into the widest range of difficult-to-transfect and common cell types with improved cell viability.

Lipofectamine 2000

Lipofectamine 2000 Reagent is a proprietary formulation that facilitates highly efficient delivery of short interfering RNA (siRNA) or plasmid DNA to mammalian cells.

Cotransfection Protocol with Lipofectamine 2000

This reference provides general guidelines and a procedure to cotransfect plasmid DNA and an RNAi molecule (i.e. RNAi, siRNA, shRNA plasmid or miRNA plasmid) into mammalian cells using Lipofectamine™ 2000 Reagent are described in this section.