General Procedures and Protocols for Using CaptureSelect Affinity Products

General affinity procedures

A detailed protocol is designed for the use of each CaptureSelect affinity product. Each product group is designed to work with a range of standard applications. POROS CaptureSelect analytical columns are designed for rapid quantitation and small-scale sample preparation of immunoglobulins, fusion proteins, antibody fragments (Fabs), and bispecific antibodies. Antibody Toolbox products are used for the purification of antibodies and antibody fragments from serum, plasma, and cell culture supernatants.

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Proteomics Toolbox products are used to improve proteomics analysis by depleting human plasma of selected abundant proteins. Biotinylated CaptureSelect ligands are used for the detection, quantitation, and characterization of specific antibodies in applications such as capture ELISA and other immunoassays, and in label-free detection platforms like bio-layer interferometry (BLI) and surface plasmon resonance (SPR). CaptureSelect protein purification products are used for the detection and purification of proteins from recombinant sources.

CaptureSelect Affinity Protocols

Here is a complete list of the current products, with general protocol links:

 

Abbreviations: Hu, human; Ig, immunoglobulin; LC, light chain; MS, multi-species; mur, murine; ung, ungulate.