NucRed Dead 647 ReadyProbes Reagent Protocol
Membrane integrity—based viability assay
NucRed Dead 647 reagent is a DNA stain that labels dead or dying cells, but does not enter living cells.
This protocol can be used for:
- Identifying dead cells using a fluorescence microscope
This protocol should not be used for:
- Flow cytometry
|1. Culture cells in appropriate medium and vessel for microscopy
|2. Add 2 drops NucRed Dead 647 reagent per milliliter of medium to label cells
|3. Incubate 5–15 minutes
|4. Image cells
Spectral information and storage
|NucRed Dead 647 ReadyProbes Reagent|
|Standard filter set||Cy5|
|EVOS Light Cube||Cy5|
|Storage conditions||Room temperature|
- In some cases, more or fewer drops may be needed to achieve optimal staining intensity
- Image quality may be improved by replacing medium with Live Cell Imaging Solution (A14291DJ)
HeLa cells stained with NucRed Dead 647 ReadyProbes Reagent.
For Research Use Only. Not for use in diagnostic procedures.