sho-readyprobes-red

Membrane integrity—based viability assay

NucRed Dead 647 reagent is a DNA stain that labels dead or dying cells, but does not enter living cells.

This protocol can be used for:

  • Identifying dead cells using a fluorescence microscope

This protocol should not be used for:

  • Flow cytometry

You will need the following for this protocol:

Protocol

1. Culture cells in appropriate medium and vessel for microscopy
2. Add 2 drops NucRed Dead 647 reagent per milliliter of medium to label cells
3. Incubate 5–15 minutes
4. Image cells

Spectral information and storage

  NucRed Dead 647 ReadyProbes Reagent
Excitation/Emission 642/661 nm
Standard filter set Cy5
EVOS Light Cube Cy5
Storage conditions Room temperature

 

Protocol tips

Image of cells stained with NucRed Dead 647 ReadyProbes Reagent

HeLa cells stained with NucRed Dead 647 ReadyProbes Reagent.