Tetrahymena pyriformis staining using mouse anti beta-tubulin monoclonal antibody.

Tetrahymena pyriformis were cultured with EdU (A10044), Click-iT® GalNAz glycoprotein labeling reagent (C33365), and InSpeck™ Blue (350/440) Intensity Calibration microspheres (I7221). Following fix/perm using Image-iT™ Fixation/Permeabilization kit (R37602), EdU-incorporated DNA was labeled with Alexa Fluor® 488 azide (A10266) and GalNAz-incorporated cellular components with Alexa Fluor® 555 alkyne (A20013). Cilia were labeled with anti-beta-tubulin Ab (32-2600) and Alexa Fluor® 647 secondary Ab (A21236). Imaging followed mounting in SlowFade® Gold (S36937).

<i>Tetrahymena pyriformis</i> were cultured with EdU (A10044), Click-iT® GalNAz glycoprotein labeling reagent (C33365), and InSpeck™ Blue (350/440) Intensity Calibration microspheres (I7221). Following fix/perm using Image-iT™ Fixation/Permeabilization kit (R37602), EdU-incorporated DNA was labeled with Alexa Fluor® 488 azide (A10266) and GalNAz-incorporated cellular components with Alexa Fluor® 555 alkyne (A20013). Cilia were labeled with anti-beta-tubulin Ab (32-2600) and Alexa Fluor® 647 secondary Ab (A21236). Imaging followed mounting in SlowFade® Gold (S36937).

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