Wound healing applications using CellTracker™ Deep Red

Neonatal human dermal fibroblasts were grown to confluency and then labeled with 1 µM CellTracker™ Deep Red for 30 minutes in Live Cell Imaging Solution (LCIS). LCIS was removed and media placed back on cells. A scratch wound was made in the cells. The entire wound was scanned using the EVOS® FL Auto System using the scan function. The same area was re-scanned 24 and 48 hours later to monitor how the wound was healing. The initial scan of the fresh wound was analyzed using the measure function on the EVOS® system to calculate the area of the wound (7.25 x 106 µm2).

Neonatal human dermal fibroblasts were grown to confluency and then labeled with 1 µM CellTracker™ Deep Red for 30 minutes in Live Cell Imaging Solution (LCIS). LCIS was removed and media placed back on cells. A scratch wound was made in the cells. The entire wound was scanned using the EVOS® FL Auto System using the scan function. The same area was re-scanned 24 and 48 hours later to monitor how the wound was healing. The initial scan of the fresh wound was analyzed using the measure function on the EVOS® system to calculate the area of the wound (7.25 x 106 µm<sup>2</sub>).

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