Peptide mapping protocols that detail the entire protein (100% sequence coverage) are required to prove molecular structure as well as determine post-translational modifications (PTMs). Complex protein digests require high peak capacity and high-resolution separations. Our peptide mapping workflow solution includes a fast, reproducible protein digestion protocol and powerful yet simple peptide mapping software, combined with the Thermo Scientific Vanquish Flex or Thermo Scientific Vanquish Horizon UHPLC system.


Peptide mapping workflow

MS/MS spectrum of the rituximab glycopeptide aa 290-302 (TKPREEQFN*STFR,*=G0) with the typical fragmentation pattern: the two oxonium ions 204 (HexNAc), 366 (Hex-Hex-NAc), and the sequence ladder of fragmented glean attached to the intact peptide.
MS/MS spectrum of the rituximab glycopeptide aa 290-302 (TKPREEQFN*STFR,*=G0) with the typical fragmentation pattern: the two oxonium ions 204 (HexNAc), 366 (Hex-Hex-NAc), and the sequence ladder of fragmented glean attached to the intact peptide.

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Peptide mapping is a routine method to characterize biopharmaceutical proteins that have been enzymatically digested resulting in complex samples. Peptide mapping methods are often developed and evaluated with combined UV and MS detection to identify, characterize, confirm and quantify the peptides. Coupling the Vanquish UHPLC with a Thermo Scientific Orbitrap mass spectrometer and Thermo Scientific BioPharma Finder software provides accurate, in-depth characterization and relative quantification of biotherapeutic peptides for streamline peptide mapping confidence. Further improve your productivity by implementing tandem LC workflows with the Vanquish Duo UHPLC using existing validated methods while reducing carryover, with extended column washes, without sacrificing throughput.


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