Choose the Orbitrap MS that's right for your laboratory

  Q Exactive Plus
Attributes Exactive Plus (EMR) Q Exactive Focus Q Exactive (Plus) Q Exactive
HF
Q Exactive
HF-X
Q Exactive
UHMR
Max resolution (FWHM) @m/z 200 140k 70k 140k
(280k Enh R)
240k 200k (at m/z 400)
Resolution - - - - - -
Mass accuracy, (internal) <1 ppm
<1 ppm
Mass accuracy (external) <5 ppm <3 ppm <3 ppm <3 ppm <3 ppm <3 ppm
Mass range 350 to 20,000 m/z 50 to 3,000 m/z 50 to 6,000 m/z 50 to 8,000 m/z 50 to 8,000 m/z 350 to 80,000 m/z
Dynamic Range >5000:1 >5000:1 >5000:1 >5000:1 >5000:1 >5000:1
Scan rates 12 Hz 40 Hz 40 Hz 12 Hz 
Polarity switch <1 sec <1 sec
PRM - Yes Yes
Multiplex (precursor/ scan) - - 10 10
Performance and Value
  Orbitrap Elite Orbitrap Fusion
Attributes Orbitrap
Elite
Orbitrap Fusion Orbitrap Fusion Lumos Orbitrap ID-X
Max resolution (FWHM) @m/z 200 340k 500k 1,000k 7.5K–500k
Mass accuracy, (internal) <1ppm

<1 ppm RMS using internal calibration

<3 ppm RMS using external calibration, 

Mass range - - - 50–2,000 m/z
Scan rates 4Hz 20Hz 40Hz

30 Hz Orbitrap mass analyzer MSn

40 Hz ion trap MS

Polarity switch Not during run < 1.1 sec 1.1 sec
PRM Yes -
Multiplex (precursor/ scan) - 10 20 10
MSn (n=10) Yes Yes
ETD option Yes -
Decision-tree (CID/HCD/ ETD) Yes -
Synch MS3 - Yes -
Performance and Value
  Q Exactive Plus
Attributes Exactive Plus (EMR) Q Exactive Focus Q Exactive (Plus) Q Exactive
HF
Q Exactive
HF-X
Q Exactive
UHMR
Max resolution (FWHM) @m/z 200 140k 70k 140k
(280k Enh R)
240k 200k (at m/z 400)
Resolution - - - - - -
Mass accuracy, (internal) <1 ppm
<1 ppm
Mass accuracy (external) <5 ppm <3 ppm <3 ppm <3 ppm <3 ppm <3 ppm
Mass range 350 to 20,000 m/z 50 to 3,000 m/z 50 to 6,000 m/z 50 to 8,000 m/z 50 to 8,000 m/z 350 to 80,000 m/z
Dynamic Range >5000:1 >5000:1 >5000:1 >5000:1 >5000:1 >5000:1
Scan rates 12 Hz 40 Hz 40 Hz 12 Hz 
Polarity switch <1 sec <1 sec
PRM - Yes Yes
Multiplex (precursor/ scan) - - 10 10
Performance and Value
  Orbitrap Elite Orbitrap Fusion
Attributes Orbitrap
Elite
Orbitrap Fusion Orbitrap Fusion Lumos Orbitrap ID-X
Max resolution (FWHM) @m/z 200 340k 500k 1,000k 7.5K–500k
Mass accuracy, (internal) <1ppm

<1 ppm RMS using internal calibration

<3 ppm RMS using external calibration, 

Mass range - - - 50–2,000 m/z
Scan rates 4Hz 20Hz 40Hz

30 Hz Orbitrap mass analyzer MSn

40 Hz ion trap MS

Polarity switch Not during run < 1.1 sec 1.1 sec
PRM Yes -
Multiplex (precursor/ scan) - 10 20 10
MSn (n=10) Yes Yes
ETD option Yes -
Decision-tree (CID/HCD/ ETD) Yes -
Synch MS3 - Yes -
Performance and Value

Definition of terms

Resolution: Frequently reported in full width at half maximum (FWHM), resolution is the separation of two mass spectrometry peaks and is calculated as the mass of a peak (M) divided by resolving power (∆M).

Mass accuracy: Expressed in parts per million, this value indicates how much an instrument deviates from a known standard or calibrant.

Scan rate: Measured in hertz, the scan rate of an MS indicates how quickly it scans a mass spectrum.

Polarity switch: The collection of both positive and negative sample ionization data (spectra) by the ion trap during a single MS analysis. Polarity switching is applied to the study of drugs, drug metabolites, pesticides, etc.

PRM: Parallel reaction monitoring (PRM) is a targeted method of high resolution MS quantification in which a full scan of each precursor ion transition is monitored; i.e., all fragments derived from the precursor ion are monitored. In contrast, single reaction monitoring (SRM) performs one transition monitoring at a time.

Multiplex: The ability of a mass analyzer to acquire data from multiple precursor ions in the same scan without compromising high signal-to-noise ratio.

MSn: Also termed tandem MS, the use of multiple stage MS to capture product ion spectra of a defined m/z, where n is the number of recorded product ion stages.

ETD: Electron transfer dissociation (ETD) results in the fragmentation of the peptide backbone of MS samples, simplifying identification of peptide modification sites.

Synchronous MS3: A method of quantitation that involves simultaneous selection of several MS2 precursors for MS3 analysis and is often used during TMT and iTRAQ experiments.


Resources

Literature

Webinars