pSilencer™ 3.1-H1 hygro Vector Map

pSilencer™ 3.1-H1 hygro Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.

pSilencer™ 3.1-H1 hygro Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'

3.0rev: 5'-GAGTTAGCTCACTCATTAGGC-3'




pSilencer™ 3.1-H1 hygro Restriction Sites (Vector Size: 4552 bp)

Enzymes with single Restriction Sites

Site Enzyme
915AflIII
1808AhdI
1331AlwNI
3661AsiSI
4062AvaI
4083AvrII
495BamHI
239BbeI
2313BcgI
2347BcgI`
4032BclI
4093BseRI
2790BsmI
3706BspMI
2736BstZ17I
4541EcoO109I
556HindIII
235KasI
2923NaeI
236NarI
2921NgoMIV
915PciI
3996PshAI
3690PstI
3612RsrII
3245SacII
2727SalI
799SapI
4315SexAI
237SfoI
3661SgfI
4064SmaI
4088StuI
4062XmaI
2407XmnI

Non-Cutting Enzymes:

Enzyme
AarI
Acc65I
AfeI
AflII
AgeI
AleI
AloI
AloI'
ApaI
AscI
BaeI
BaeI'
BanII
BbsI
BbvCI
BglII
BlpI
BmgBI
BmtI
Bpu10I
BsaBI
BsgI
BsiWI
BsrGI
BssHII
BstBI
BstEII
BstXI
Bsu36I
BtrI
ClaI
EcoICRI
EcoNI
EcoRV
FalI
FalI'
FseI
FspAI
HpaI
KpnI
MfeI
MluI
MscI
NheI
NotI
NruI
PacI
PflMI
PmeI
PpuMI
PspOMI
PsrI
PsrI'
SacI
SanDI
SbfI
SfiI
SgrAI
SnaBI
SpeI
SrfI
SwaI
TaqII'
XbaI
XcmI
XhoI