WE42245-header

Raise Standards for Preclinical Research:
Antibody Validation

Antibodies are some of the most critical research reagents used in the lab. Poor specificity or application performance can significantly frustrate the ability to obtain good results, which can cause critical delays. Underperforming antibodies result in a lack of reproducibility, wasting time and money. To help ensure superior antibody results, we've expanded our specificity testing methodology. In this seminar,  we will present our two-part approach for validation of our antibody portfolio to ensure proper characterisation and consistency for antibodies in your laboratory.

Download seminar slides 

raise-standards-thumb

Displayed on the bus

Download
Our free eBook on Antibody Validation  


Download
eLearning modules on
specificity testing 

Learn more about our 2-part testing approach at thermofisher.com/antibodyvalidation




Finding the right antibody has never been easier
Discover the new antibody shopping experience and find with ease the exact, high-quality antibody you're looking for.
thermofisher.com/antibodies

Interrogation of Three-Dimensional Cell Models in Research

Interrogation of Three-Dimensional Cell Models in Research Traditionally, attempts to characterise aberrant cellular processes underlying cancer progression and the development of targeted therapies have relied upon two-dimensional (2D) cell models. While useful, these models do not accurately recapitulate the complex environment of cells in the body, both normal and transformed.  Increased interest in adopting three-dimensional (3D) cell models which better reflect the ‘natural’ setting has stemmed from studies demonstrating improved predictive clinical value of therapeutics that are screened in 3D  versus 2D cultures. While a wide range of routine analytical methods can be performed on 3D cultures, ranging from genomic through proteomic to cell-based, fluorescence imaging in particular has lent itself to the study of these model types.  When combined with antibody-based and functional biosensors, fluorescence microscopy is able to provide readouts of biological processes and regional differences throughout the sample, such as tumour spheroids, without disruption. This seminar will focus on the advantages and challenges of both labelling and imaging of structural and functional landscapes in 3D models relevant to solid tumour cell biology.

Download seminar slides 

3d-thumb

Displayed on the bus

Learn about our products
EVOS FL Auto 2 Cell
Imaging System ›

Guided learning
Learn about imaging microscopy and high-content analysis

Watch our video
Organoid Research with
EVOS FL Auto 2 ›

Download
Download our latest 3D
imaging poster  ›

Insights into Signalling Pathways

Quantitative analysis of two signalling pathways using multiplex-immunoprecipitation, targeted mass spectrometry and western blotting. The lack of rigorously verified reagents and a reliance on semi-quantitative immunoassays limit the accurate quantitative analysis of signalling pathway proteins. Immunoprecipitation coupled with mass spectrometry (IP-MS) enables the enrichment of signalling pathway proteins, revealing protein-protein interactions and PTMs and can be used for the identification of low-abundant pathway targets and for the assessment of antibody specificity. Multiplex IP coupled with MS (mIP-MS) further enhances this workflow by simultaneously quantifying multiple proteins and their phosphorylation states in a specific signalling pathway. mIP-tMS assays allowed absolute quantitation of multiple total and phosphorylated targets from signalling pathways in low to sub-nanogram concentrations.

Download seminar slides ›

signalling-pathways-thumb

Displayed on the bus

Learn about our products
Invitrogen iBright Imaging
Systems 

Download
Free technical handbook for mass spectrometry 

Download
Your copy of the Interactive Western Blot Guide ›

Leveraging Animal Models for Research

With the growing adoption of personalised medicine, recent advances in large scale and high throughput cellular analyses are helping streamline tools that make it easier to attain markers of disease status, examine tumour micro-environments at the single cell level and make for broad biomarker discovery and validation. Researchers today are seeking answers to questions regarding specific attributes of a tumour type, specific tumour cells and tumour micro-environment that are predictive of response in order to explore the immune system and identify pharmacodynamic biomarkers. This seminar focuses on addressing the common challenges cancer researchers face when working in animal models, and discuss recent advances and future prospects.

Download seminar slides  ›

using-animal-thumb

Displayed on the bus

Learn about our products
2018 Attune NxT Flow Cytometer 

Review our guide
Guide: Consideration when Purchasing a Flow Cytometer 

Watch our latest product video
Robotic Automation for
Flow Cytometry ›


Read our Rare
Events Book

Handbook: Detecting rare
events using flow cytometry: 
a step-by-step guide ›

Raise Standards for Preclinical Research:
Antibody Validation

Antibodies are some of the most critical research reagents used in the lab. Poor specificity or application performance can significantly frustrate the ability to obtain good results, which can cause critical delays. Underperforming antibodies result in a lack of reproducibility, wasting time and money. To help ensure superior antibody results, we've expanded our specificity testing methodology. In this seminar,  we will present our two-part approach for validation of our antibody portfolio to ensure proper characterisation and consistency for antibodies in your laboratory.

Download seminar slides 

raise-standards-thumb

Displayed on the bus

Download
Our free eBook on Antibody Validation  


Download
eLearning modules on
specificity testing 

Learn more about our 2-part testing approach at thermofisher.com/antibodyvalidation




Finding the right antibody has never been easier
Discover the new antibody shopping experience and find with ease the exact, high-quality antibody you're looking for.
thermofisher.com/antibodies

Interrogation of Three-Dimensional Cell Models in Research

Interrogation of Three-Dimensional Cell Models in Research Traditionally, attempts to characterise aberrant cellular processes underlying cancer progression and the development of targeted therapies have relied upon two-dimensional (2D) cell models. While useful, these models do not accurately recapitulate the complex environment of cells in the body, both normal and transformed.  Increased interest in adopting three-dimensional (3D) cell models which better reflect the ‘natural’ setting has stemmed from studies demonstrating improved predictive clinical value of therapeutics that are screened in 3D  versus 2D cultures. While a wide range of routine analytical methods can be performed on 3D cultures, ranging from genomic through proteomic to cell-based, fluorescence imaging in particular has lent itself to the study of these model types.  When combined with antibody-based and functional biosensors, fluorescence microscopy is able to provide readouts of biological processes and regional differences throughout the sample, such as tumour spheroids, without disruption. This seminar will focus on the advantages and challenges of both labelling and imaging of structural and functional landscapes in 3D models relevant to solid tumour cell biology.

Download seminar slides 

3d-thumb

Displayed on the bus

Learn about our products
EVOS FL Auto 2 Cell
Imaging System ›

Guided learning
Learn about imaging microscopy and high-content analysis

Watch our video
Organoid Research with
EVOS FL Auto 2 ›

Download
Download our latest 3D
imaging poster  ›

Insights into Signalling Pathways

Quantitative analysis of two signalling pathways using multiplex-immunoprecipitation, targeted mass spectrometry and western blotting. The lack of rigorously verified reagents and a reliance on semi-quantitative immunoassays limit the accurate quantitative analysis of signalling pathway proteins. Immunoprecipitation coupled with mass spectrometry (IP-MS) enables the enrichment of signalling pathway proteins, revealing protein-protein interactions and PTMs and can be used for the identification of low-abundant pathway targets and for the assessment of antibody specificity. Multiplex IP coupled with MS (mIP-MS) further enhances this workflow by simultaneously quantifying multiple proteins and their phosphorylation states in a specific signalling pathway. mIP-tMS assays allowed absolute quantitation of multiple total and phosphorylated targets from signalling pathways in low to sub-nanogram concentrations.

Download seminar slides ›

signalling-pathways-thumb

Displayed on the bus

Learn about our products
Invitrogen iBright Imaging
Systems 

Download
Free technical handbook for mass spectrometry 

Download
Your copy of the Interactive Western Blot Guide ›

Leveraging Animal Models for Research

With the growing adoption of personalised medicine, recent advances in large scale and high throughput cellular analyses are helping streamline tools that make it easier to attain markers of disease status, examine tumour micro-environments at the single cell level and make for broad biomarker discovery and validation. Researchers today are seeking answers to questions regarding specific attributes of a tumour type, specific tumour cells and tumour micro-environment that are predictive of response in order to explore the immune system and identify pharmacodynamic biomarkers. This seminar focuses on addressing the common challenges cancer researchers face when working in animal models, and discuss recent advances and future prospects.

Download seminar slides  ›

using-animal-thumb

Displayed on the bus

Learn about our products
2018 Attune NxT Flow Cytometer 

Review our guide
Guide: Consideration when Purchasing a Flow Cytometer 

Watch our latest product video
Robotic Automation for
Flow Cytometry ›


Read our Rare
Events Book

Handbook: Detecting rare
events using flow cytometry: 
a step-by-step guide ›