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Stay up to date with all our electron microscopy product launches, resources, application technique discussions, and maintenance notices.
Featured articles
Talos 12 TEM: The next step in 120 kV imaging
The Thermo Scientific Talos 12 TEM is a modern 120 kV transmission electron microscope designed as an upgrade path from legacy TEM systems. It delivers improved optical performance, faster data acquisition, and a more streamlined, user-friendly experience, while adding robust stability and digital, automation-ready control for long-term productivity.
- Modern digital control and automation
- Outstanding optical performance
- Designed for ease of use and shared labs
- Smart upgrade from legacy TEMs
100 kV TEM for high-resolution cryo-EM? Absolutely.
This paper, featured on the cover of Structure, shows how the 100 keV Thermo Scientific Tundra Cryo-TEM with a Falcon C Direct Electron Detector can deliver high-resolution single-particle cryo-EM, including sub-3 Å reconstructions, challenging the idea that lower-voltage TEMs are mainly for screening.
Key results:
- Demonstrated structures across a wide size range (~50–55 kDa to 3.9 MDa) using the 100 keV setup
- Reported example resolutions: apoferritin 2.1 Å, T20S proteasome 2.7 Å, GABA_A receptor 2.8 Å, AAV9 capsid 2.8 Å (and additional mid-resolution cases like hemoglobin 5.0 Å and transthyretin 3.5 Å)
By combining improved source/optics, higher detector performance, and reduced contamination/drift, the work argues that 100 keV cryo-TEMs can be viable for routine, high-resolution structure determination, potentially lowering cost/complexity barriers for labs adopting cryo-EM.
Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii
Researchers report an open, community resource for visual proteomics: 1,829 annotated in situ cryo-electron tomography (cryo-ET) tomograms plus raw data from the green alga Chlamydomonas reinhardtii. Using cryo-plasma FIB milling and modern cryo-ET workflows, they capture detailed views of diverse eukaryotic organelles and benchmark dataset quality by subtomogram averaging of native complexes (including ribosomes, Rubisco, nucleosomes, microtubules, clathrin, photosystem II, and ATP synthase), with many reconstructions reaching sub-nanometer resolution. The dataset is designed to accelerate both method development (segmentation, averaging, analysis) and biological discovery by making large-scale, high-quality cryo-ET data broadly reusable.
Service spotlight
Maintain peak electron microscope performance with genuine OEM parts.
Designed for perfect fit and reliability, OEM parts deliver proven quality, long-term cost efficiency, and optimal performance, helping to reduce downtime, protect your equipment, and keep productivity at its best.
- Reliable fit and compatibility—OEM parts are engineered to fit perfectly, ensuring seamless operation and reducing the risk of malfunctions.
- Proven quality—Rigorously tested OEM parts meet the highest quality standards for dependable performance in all conditions.
- Long-term cost savings—Durable and reliable OEM parts reduce replacements and lower overall maintenance costs.
- Peak performance—OEM parts help maintain optimal performance, minimizing downtime and maximizing productivity.
Optimizing electron microscope performance with advanced remote technical support
In today's fast-paced technological environment, the need for efficient, reliable, and expert technical support is more critical than ever. Fully integrated into our processes and backed by an advanced suite of digital tools, our Remote Technical Support (RTS) is an essential part of a modern service ecosystem, resolving technical issues promptly, minimizing downtime, and optimizing productivity.
RTS leverages advanced digital tools and technologies to enhance our service delivery:
- Diagnostic software
- Communication platforms
- AI-enhanced knowledgebase
RTS teams use a range of secure screen-sharing, remote access, communication, and diagnostic tools to quickly understand and resolve your issues. By selecting the most appropriate option for each situation, they ensure fast, accurate, and customer-focused support. Read more about select tools used to help resolve issues and keep you productive.
Software Updates
Amira Software 2025.1
Thermo Scientific Amira Software 2025.1 brings major workflow and visualization upgrades for EM and 3D analysis. This release introduces next-level 3D rendering with an overhauled lighting system (environment mapping + realistic materials) for more informative, presentation-ready visuals. New 2.5D segmentation preserves structural continuity by adding depth-aware context while keeping resource use low, and AI-Assisted Selection speeds up accurate annotation of structures of interest. AI segmentation is also more efficient, delivering ~85% faster training through dynamic augmentation routines. Rounding out the update is a new Reporting workroom with a modern charting library for clearer data visualization, plus new Xtras including CellposeSAM for robust instance segmentation across modalities and AI-based object classification for automated categorization and analysis.
Smart EPU v3.15 and CryoTomo SW Suite v3.25
Gain access to new functionalities in our software for our cryo-EM workflows:
EPU Software 3.15 brings expanded compatibility and faster, more efficient automated sample screening
The latest release of Thermo Scientific EPU Software enhances the Center Beam Option during Automated Acquisition, extending support to a wider range of grids and microscope systems for higher data-quality yield. Smart Screening performance is improved, with faster run completion through reduced stage movement, helping you collect high-quality data more efficiently.
CryoTomo Software Suite 3.25 enhances usability and efficiency in cryo-ET data collection
This release of Thermo Scientific CryoTomo Software Suite introduces custom naming for Search Maps, improving data organization and traceability throughout the workflow. Tomogram acquisition is now more efficient across different lamella pre-tilts, with automatic skipping of stage-inaccessible angles and clear user notifications. Together, these improvements streamline acquisition, reduce unnecessary steps, and help users focus on collecting the most relevant data.
More information about these and other applications can be found in our Software Resource Portal.
Resources
Image Analysis of Electron Tomography in Cell Biology
Learn how to tackle common image-processing hurdles in electron tomography (ET) workflows—across both cryo-ET and room-temperature experiments. This on-demand session covers practical approaches for noise reduction, alignment/reconstruction, contrast enhancement, segmentation, artifact removal, and data management to help you achieve clearer, more accurate imaging results. You’ll also hear key takeaways on improving image quality and filament detection in cryo-ET, using AI to streamline segmentation of complex biological structures, and combining light and electron microscopy via CLEM for more comprehensive cell analysis.
Webinar: Streamlining Cryo-EM for Tomography and Single Particle Analysis Workflows
Learn how recent advances in cryo-electron microscopy are accelerating structural insights, from atomic-resolution single-particle analysis (SPA) to in situ cryo-electron tomography (cryo-ET), and helping connect key steps across the workflow. In this session, our experts highlight four Thermo Scientific innovations designed to improve efficiency, performance, and connectivity between cryo-FIB and cryo-TEM workflows, including high-throughput lamella prep, contamination-minimized transfer, faster data collection, and workflow automation with the CryoTomo Software Suite.
Ebook: Examining cells and organelles in 3D with the high resolution of cryo-electron tomography
This eBook explains how cryo-electron tomography (cryo-ET) helps cell biologists bridge the gap between molecular detail and whole-cell context by visualizing organelles and macromolecular structures in situ, in a near-native frozen-hydrated state, and in 3D, without staining or labeling. You will get a practical tour of what cryo-ET is, where it fits alongside other imaging modalities, and a typical end-to-end workflow.
What you’ll learn:
- Why cellular context matters and which biological questions cryo-ET can uniquely answer
- How cryo-ET enables quantitative insights through segmentation and analysis
- How advances in automation and workflows are helping scale cryo-ET for statistically powerful studies
For Research Use Only. Not for use in diagnostic procedures.