Sample Prep Automation Survey | Thermo Fisher Scientific

1. What are the 3 main reasons you or your lab use automation? (Check top 3)

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Improved reproducibility of results and less variation in each run

Improved sample throughput

Less hands-on time

More cost-effective

Easier to use than manual protocol

Other

2. Which instrument do you use to automate steps of your sample preparation? If more than one, please select those most commonly used

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KingFisher Instruments from Thermo Fisher Scientific

QIAcube™ from Qiagen

Maxwell™ from Promega

MultiMACS™ M96/M96thermo Separators from Miltenyi Biotec

Other

1. What do you see as the main 3 benefits of automation? (Check top 3)

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Improved reproducibility of results and less variation in each run

Improved sample throughput

Less hands-on time

More cost-effective

Easier to use than manual protocol

Other

2. What do you see as the main 3 challenges or concerns with automation for sample prep? (Check top 3)

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Expense of instrument

Expensive reagents/run-rate cost

Time-consuming or complex to set up and maintain

Possibility of instrument error or failure

Loss of yield, purity, or sensitivity when automating

Primarily benefits high-throughput customers

Availability of automated protocols for the products I use

Other

3. What are the main reasons your lab has not yet purchased an instrument for automated sample prep?

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Not sufficient throughput to justify the cost

Instruments are too expensive

The reagents are too expensive

Don’t know what to choose

Don’t have the time to investigate it

Afraid of losing yield, purity, or sensitivity when automating

Other

5. Which sample prep purifications do you perform? Check all that apply.

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RNA

DNA

Protein

Cells

Exosomes

Bacteria

Other

6. Which type of analyte do you prep most frequently? Check all that apply:

gDNA

Cell free DNA

vDNA

mRNA

Total RNA (no miRNAs)

True total RNA (with miRNAs)

Cell free RNA

Protein bound to DNA—Chromatin immunoprecipitation (ChIP)

Protein bound to RNA—RNA immunoprecipitation (RIP)

Protein-protein interactions (co-IP)

Single proteins/peptides

For cells, bacteria, or other, please specify

7. Which type of reagents/kits do you use for your sample purification?

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Magnetic-bead based kits

Spin column kits

Other

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