The Human c-Peptide ELISA quantitates Hu c-Peptide in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu c-Peptide.
Principle of the method
The Human c-Peptide solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
C-peptide, also called connecting peptide, is a polypeptide that connects the insulin A and B chain. Its measurement in diabetes can help to distinguish between certain diseases with the same clinical features.
In vivo studies with animal with diabetes 1 showed that administration of C-peptide leads to improvement in nerve and kidney function. It has been observed that C-peptide also has anti-inflammatory effects and plays an important role in the emergency repair of smooth muscle cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.