Invitrogen
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Analytical sensitivity
1.7 ng/mL
Assay range
3.1-50 ng/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Interassay CV
6%
Intraassay CV
6.2%
Instrument
Colorimetric Microplate Reader
Product size
96 tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Storage
2-8°C
Protein name
Cathepsin L
Protein aliases
Cathepsin L, Major excreted protein, MEP, Cathepsin L1, Cathepsin L1 heavy chain, Cathepsin L1 light chain
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
CATL, CTSL, CTSL1, MEP
Gene ID
Gene symbol
CTSL
UniProt ID
The Human Cathepsin L ELISA quantitates Hu Cathepsin L in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Cathepsin L.
Principle of the method
The Human Cathepsin L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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