Analytical sensitivity
2.67 pg/mL
Assay range
62.5-2000 pg/mL
Sample type/volume
Cell Lysate
12.5 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr
Homogenous (no wash)
No
Interassay CV
6.3%
Intraassay CV
3.7%
Instrument
Colorimetric Microplate Reader
Product size
96 tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Detection Antibody
anti rabbit-HRP
Cell Lysis Buffer
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Storage
2-8°C
Protein name
ERK1/2
Target site-specificity
Phosphorylated
Thr202
Phosphorylated
Tyr204
Protein aliases
ERK-1, ERK-2, ERT1, ERT2, Extracellular signal-regulated kinase 1, Extracellular signal-regulated kinase 2, extracellular signal-related kinase 1, extracellular-signal-regulated kinase 2, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 2, MAP kinase 3, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, MAPK 3, Microtubule-associated protein 2 kinase, mitogen activated protein kinase 1, mitogen-activated 3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 3, MNK1, p42-MAPK, p44 MAP kinase, p44-ERK1, p44-MAPK, pp42/MAP kinase, protein tyrosine kinase ERK2
Protein family
Other Proteins
Species (tested)
Human, Mouse, Rat
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
Gene aliases
9030612K14Rik, AA407128, AU018647, C78273, ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, Esrk1, HS44KDAP, HUMKER1A, Mapk, MAPK1, MAPK2, MAPK3, MNK1, Mtap2k, p38, p40, p41, p41mapk, p42-MAPK, P42MAPK, p44, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM1, PRKM2, PRKM3
Gene symbol
MAPK1, MAPK3, Mapk1, Mapk1, Mapk3, Mapk3
UniProt ID
The ERK1/2 [pThr202/Tyr204] ELISA quantitates IL-1b in cell lysates. The assay will exclusively recognize both natural and recombinant ERK 1/2, when phosphorylated at Threonine 202 and Tyrosine 204.
Principle of the method
The phospho ERK1/2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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