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ERK1/ERK2 (Phospho) [pT202/pY204]/[pT185/pY187] Multispecies InstantOne™ ELISA Kit
![ERK1/ERK2 (Phospho) [pT202/pY204]/[pT185/pY187] Multispecies InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/650/erk-datafile-phosp-pj-tds.jpg?time=20220826 "ERK1/ERK2 (Phospho) [pT202/pY204]/[pT185/pY187] Multispecies InstantOne™ ELISA Kit")
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ERK1/ERK2 (Phospho) [pT202/pY204]/[pT185/pY187] Multispecies InstantOne™ ELISA Kit
Representative data for phosphoERK1/2 InstantOne™ ELISA. A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor), washed and unstimulated (-) or stimulated with 10 ng/mL EGF for 10 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the bands at 44 and 42 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Storage
2-8°C
Protein name
ERK1/2
Target site-specificity
[Phosphorylated Thr202, Phosphorylated Tyr204, Phosphorylated Thr185, Phosphorylated Tyr187]
Protein aliases
ERK-1, ERK-2, ERT1, ERT2, Extracellular signal-regulated kinase 1, Extracellular signal-regulated kinase 2, extracellular signal-related kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 2, MAP kinase 3, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 3, p42-MAPK, p44-ERK1, p44-MAPK, protein tyrosine kinase ERK2
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, HS44KDAP, HUMKER1A, MAPK1, MAPK2, MAPK3, p38, p40, p41, p41mapk, p42-MAPK, P42MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM1, PRKM2, PRKM3
Gene ID
Gene symbol
MAPK1, MAPK3
UniProt ID
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster ERK 1/2 in cell lysates. Detection of rat ERK 1/2 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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