Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
96 tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Storage
2-8°C
Protein name
GSK3B
Target sub-specificity
Total
Protein aliases
Glycogen synthase kinase-3 beta, GSK-3 beta, Serine/threonine-protein kinase GSK3B, GSK3beta isoform
Species (tested)
Human, Mouse
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
7330414F15Rik, 8430431H08Rik, C86142, GSK-3, GSK-3beta, GSK3, GSK3B
Gene ID
Gene symbol
GSK3B, Gsk3b
UniProt ID
The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse GSK3b (Ser9) in cell lysates. Detection of total and phosphorylated rat GSK3b (Ser9) is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Glycogen synthase kinase-3 beta (GSK3 beta) belongs to the subfamily of glycogen synthase kinases, that play a central role in energy metabolism, neuronal cell development, and body pattern formation. GSK3 has alpha and beta isoforms (51 kDa and 47 kDa respectively), that are functionally dissimilar. GSK3 beta plays a role in insulin and Wnt signaling, and is inhibited following the extracellular signaling events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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