epididymis luminal secretory protein 52, epididymis secretory sperm binding protein Li 65p, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, alpha-like 4, heat shock 90kD protein, heat shock 90kDa protein 1, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein 90kDa alpha family class A member 1, Heat shock protein HSP 90-alpha, HSP 86, LAP-2, Lipopolysaccharide-associated protein 2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38
The Human Heat Shock Protein 90 alpha (Hu HSP90 alpha) ELISA quantitates Hu HSP90 alpha in human serum, plasma cell lysate. The assay will exclusively recognize both natural and recombinant Hu HSP90 alpha.
Principle of the method
The Human HSP90 alpha solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.