Invitrogen
Analytical sensitivity
1.6 pg/mL
Assay range
1.6-100 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Hands-on time
24 hr
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 Tests
Contents
Capture (coating) Antibody
Detection Antibody
Standard
SAV-HRP
Sample Diluent
Storage
2-8°C
Protein name
IL-4
Protein aliases
B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1, IL-4, Interleukin-4, Lymphocyte stimulatory factor 1
Species (tested)
Rat
Assay kit format
Matched Antibody Pair
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
Il-4, Il4, Il4e12
Gene ID
Gene symbol
Il4
UniProt ID
The Rat IL-4 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, sample diluent and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of rat IL-4. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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