The Human Interleukin-6 Receptor (Hu IL-6R) ELISA quantitates Hu IL-6R in human serum, plasma, amniotic fluid, urine or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6R.
Principle of the method
The Human IL-6R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL6R (interleukin-6 receptor subunit alpha) is a part of the receptor for interleukin 6. The IL-6 receptor complex is composed of two membrane glycoproteins: the low affinity receptor and the signaltransducing component. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.