breast normal epithelial cell associated serine protease, kallikrein 10 protein 1, kallikrein 10 protein 12, kallikrein 10 protein 13, kallikrein 10 protein 2, kallikrein 10 protein 3, kallikrein 10 protein 4, kallikrein 10 protein 5, kallikrein 10 protein 7, kallikrein 10 protein 8, kallikrein 10 protein 9, Kallikrein-10, kallikrein-related peptidase 10, Normal epithelial cell-specific 1, Protease serine-like 1, protease, serine-like, 1
The Human Kallikrein 10 (Hu KLK10) ELISA quantitates Hu KLK10 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu KLK10.
Principle of the method
The Human KLK10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.