The Rat RAGE (AGER) ELISA quantitates Rt RAGE in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt RAGE.
Principle of the method
The Rat RAGE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.