The Human Transforming Growth Factor (TGFα) ELISA quantitates Hu TGFα in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TGFα.
Principle of the method
The Human TGFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Transforming growth factor (TGF) alpha is a 50-aa single-chain polypeptide derived from cleavage of transmembrane pro-TGF alpha. Sharing 42% identity with epidermal growth factor (EGF), TGF alpha binds the EGF receptor and activates downstream signaling pathways. TGF alpha is expressed by activated macrophages, keratinocytes, epithelial cells, and a variety of tumor-derived and transformed cell lines. This growth factor mediates cell proliferation and migration, as well as cellular transformation. Overexpression of TGF alpha has been linked to a variety of cancers, including the breast, colon, and stomach.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.