Invitrogen
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Analytical sensitivity
6.6 pg/mL
Assay range
31.0-1,000 pg/mL
Sample type/volume
Serum
10 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
5 hr 35 min
Homogenous (no wash)
No
Interassay CV
10%
Intraassay CV
10%
Instrument
Colorimetric Microplate Reader
Product size
96 tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Storage
2-8°C
Protein name
TGF beta-2
Protein aliases
BSC-1 cell growth inhibitor, Cetermin, G-TSF, Glioblastoma-derived T-cell suppressor factor, LAP, Latency-associated peptide, polyergin, prepro-transforming growth factor beta-2, TGF-beta-2, Transforming growth factor beta-2, Transforming growth factor beta-2 proprotein, transforming growth factor, beta 2
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
LDS4, TGF-beta2, TGFB2
Gene ID
Gene symbol
TGFB2
UniProt ID
The Human Transforming Growth Factor Beta 2 (TGFβ2) ELISA quantitates Hu TGFβ2 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TGFβ2.
Principle of the method
The Human TGFβ2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Golgi Protein, 58K is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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