Invitrogen
TIM-1 (HAVCR1) Human ELISA Kit
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Analytical sensitivity
2 pg/mL
Assay range
12.29-3000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatants
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Storage
2-8°C
Protein name
KIM-1
Protein family
Other Proteins
UniProt ID
O43656,Q96D42
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
CD365, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1
Gene ID
26762
Gene symbol
HAVCR1
The Human TIM-1 (HAVCR1) ELISA quantitates Hu TIM-1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TIM-1.
Principle of the method
The Human TIM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
HAVCR1, a 359 A.A Class 1 integral membrane glycoprotein, is used by Hepatitis A virus to infect humans. It consists of a conserved N-terminal Cys-rich region, IgV (Immunoglobulin Variable region like) domain and a TSP-rich mucin domain in the extracellular region followed by a highly conserved tyrosine kinase phosphorylation motif in the cytoplasmic region. HAVCR1 is expressed on terminally differentiated CD4+ Th2 cells and regulates cytokine production. It plays an important role in the regulation of immune responses in the development of asthma and allergic responses. Its expression is critically upregulated in dedifferentiated kidney cells, thus indicating a possible role in regeneration of proximal tubule epithelium in Kidneys. HAVCR1 has shown to be upregulated in renal carcinomas, suggesting its role in tumor progression and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ immunoassay does not perform as described on our data sheet, we will replace the product at no cost to you, or if you prefer, we will provide you with a credit for future purchase.*
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