Cryo-Electron Tomography

Cryo-electron tomography (cryo-ET) enables three-dimensional visualization of cells, organelles and macromolecular complexes in their near-native state. By combining cryogenic preservation with tilt-series acquisition and computational reconstruction, cryo-ET bridges cellular context with molecular resolution, revealing biological architecture as it exists in situ.

As the field advances, cryo-ET is evolving from highly specialized experiments toward more automated, repeatable and higher-throughput structural investigations. Innovations in cryo-focused ion beam (cryo-FIB) milling of cells and tissues, increasingly streamlined tomography data acquisition, and faster, more sophisticated image processing workflows are helping to improve reproducibility, efficiency and accessibility.

Continued innovation in life science microscopy, spanning advanced technologies, precision engineering and integrated workflows, is enabling this transformation and this is expanding the impact of cryo-ET across structural biology, cell biology and drug discovery research worldwide.