Thermo Fisher Scientific at CYTO 2026

Cellular analysis is greatly enabled by spatial resolution by offering useful context at the cellular and tissue levels. This tutorial highlights how the Invitrogen Attune CytPix Flow Cytometer and the Invitrogen EVOS S1000 Spatial Imaging System help provide richer single cell-level information for better biological insights. While both technologies provide single-cell resolution, the Attune CytPix platform enables high throughput and AI-assisted management of imaging data and the EVOS S1000 system provides contextual information regarding the tissue microenvironment.

The Attune CytPix flow analyzer combines fluorescence, light scatter, and brightfield imaging to integrate quantitative morphometric features with phenotypic markers, enabling data interpretation in the context of cellular morphology and physiology. This utilization of Imaging-Enhanced Flow Cytometry (IEFC) strengthens confidence in cell identity and analytical decisions. User-trainable AI models allow researchers to tailor image analysis to their own samples, converting imaging data into robust, decision-ready metrics. Integrating morphology with fluorescence, alongside tools such as image similarity and dimensionality reduction, sharpens interpretation and strengthens analytical confidence.

Extending analysis beyond single-cell suspensions, the EVOS S1000 Spatial Imaging System delivers advanced multiplexed spectral imaging of intact tissue sections within a streamlined and accessible workflow. Multiple markers can be analyzed simultaneously on a single slide, with on-the-fly spectral unmixing that simplifies imaging and reduces post-acquisition processing. We will illustrate how multiplex spatial phenotyping reveals the localization and organization of immune and tumor cells, helping to provide critical context that cannot be obtained from flow cytometry alone.

Together, these technologies underscore how single-cell precision and spatial biology, whether from IEFC or multiplex fluorescence imaging, can be aligned to help provide complementary insights for a more complete understanding of complex biology.