Nano, Capillary, and Microflow UHPLC Systems

Ideal for low-flow LC-MS workflows

Do you use applications requiring high sensitivity such as proteomics, metabolomics, and intact protein analysis? The Thermo Scientific EASY-nLC 1200 system provides effortless nanoflow UHPLC, and the Thermo Scientific UltiMate 3000 RSLCnano system provides fully integrated nano-, capillary-, and micro-flow solutions.

The right solution for every low-flow application

For scientists who have restricted sample volumes and require high sensitivity and unsurpassed retention time precision, these low flow liquid chromatography (LC) technologies are the best solution. They use the latest technologies to provide you reliable nano-, capillary-, or micro-flow LC.

Our systems are equipped with easy-to-use Thermo Scientific nanoViper Fingertight Fittings, which allow you to quickly connect LC modules, valves, and columns without additional tools, for leak-free connections that can improve chromatographic results by minimizing dead volume.

Both the EASY-nLC 1200 system and the UltiMate 3000 RSLCnano system integrate seamlessly with Thermo Scientific mass spectrometers and EASY-Spray products for plug-and-spray simplicity.

Choose from two UHPLC systems

A flexible, customizable system that meets all low flow applications spanning nano-, capillary-, and micro-flow needs. The UltiMate 3000 RSLCnano system provides excellent retention time precision for more confidence in your results.

 UltiMate 3000 RSLCnano brochure

A compact, integrated solution for nano LC-MS analyses, the EASY-nLC 1200 offers effortless nano-flow separations and is designed for intuitive operation with excellent performance. This system can handle ultra-long columns with its high pressure capability.

 EASY-nLC 1200 brochure

Systems comparison

    EASY-nLC 1200

EASY-nLC 1200

Operational simplicity and excellent performance
UltiMate 3000 RSLCnano

UltiMate 3000 RSLCnano

Versatility and uncompromising precision


System pressure
Retention time precision
Splitless nano flow    
Nano flow range Recommended range:
Recommended range:
Capillary flow range Recommended range:
Micro flow range Recommended range:

System Features

Design Integrated Modular
Column compartment n.a.*
*Built-in heating with
EASY-Spray columns
Sample capacity
Sample cooling    
nanoViper fittings    
Zero-loss sample pick-up    
Nano flow pump High pressure mixing binary syringe pump High pressure mixing binary continuous flow pump
Analytical flow and loading pump Low pressure mixing ternary gradient pump

Application Setups

Direct injection    
Preconcentration Vented column setup Continuous direct flow by integrated loading pump
2D salt setups  
Automated offline 2D LC*  
Offline fractionation*  
Tandem LC*  
*requires additional equipment


Software Xcalibur, LC Devices,
& Tracefinder
SII with Xcalibur & Tracefinder
Chromeleon Chromatography
Data System
Integrated PC  
Audit trail  


MS sources for MS integration EASY-Spray Source
Nanospray Flex Ion Source
EASY-Spray Source
Nanospray Flex Ion Source
Ion Max Ion Source
Columns EASY-Spray C18 Columns
Integrated column/emitter design minimizing dead volume.

Acclaim PepMap 100 C18 Columns
Acclaim PepMap 300 C18 Columns
Columns ideal for peptide and protein separations with inner
diameters between 50μm to 1mm

Any of our HPLC and UHPLC column
= Not applicable = Poor = Good = Better = Best

Discover the best HPLC systems for your applications and budget

Discover the best HPLC system for your applications and budget

Use these 2 tools to help you decide

HPLC Systems Selection Guide
Use this tool to rank the top HPLC instruments for your applications based on your responses to simple pull-down menus.

HPLC System Total Cost of Ownership Calculator
Use this tool to estimate your cost for purchasing and running Thermo Scientific HPLC and UHPLC systems. You can compare costs between instruments.

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Connect with Thermo Scientific mass spectrometers for outstanding specificity and sensitivity.

  • Triple quadrupole LC-MS
  • Orbitrap LC-MS
  • Ion Trap LC-MS
  • Single quadrupole LC-MS

Learn about our LC-MS options

  • Connect your lab to boost productivity with secure remote access to your data and instruments
  • Monitor your instruments in real time with automatic email notifications
  • Store data in secure accounts and share with colleagues and collaborators

Learn more

The AppsLab Library of Analytical Applications is a fully searchable online, analytical method repository where you can find applications with detailed method information, chromatograms and related compound information.

Go to AppsLab

Optimize nanoflow LC/MS for proteomics applications with Thermo Scientific EASY-Spray C18 LC Columns.

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Featured webinar
Raise your nano LC-MS performance with new Thermo Scientific solutions

In this webinar, scientists discuss the latest innovations in nano LC-MS technology with overviews of the EASY-nLC 1200 and versatile UltiMate 3000 RSLCnano systems.

 Watch webinar