Maximize results with the proven performance of CE and the power of NGS
At Thermo Fisher Scientific, we’re committed to helping you get more answers from your samples with our integrated capillary electrophoresis–next-generation sequencing (CE-NGS) forensic workflow solutions. Whether you're processing urgent request cases, routine samples, or highly degraded samples, we provide comprehensive DNA analysis solutions to the forensic community.
Whether you’re analyzing a high-priority, challenging, or routine sample, you can count on our 3,700+ sales, service, and technical support specialists to provide answers that add simplicity, scalability, and speed to your workflow—enabling you to analyze your most difficult cases with complete confidence.
Identification of human remains from rebel’s graves: CE and NGS analyses
Forensic scientists at the State Forensic Medicine Service are working to solve one of Lithuania’s greatest historical mysteries—identifying the remains from an 1863 uprising. Marija Caplinskiene, MD, PhD, discusses how her team is using both capillary electrophoresis and next-generation sequencing to analyze DNA from recovered bones found in recently revealed graves.
Forensic DNA analysis of highly degraded samples
From understanding how body decomposition affects forensic DNA analysis to simulating IED explosions with the goal of finding touch amounts of DNA, Sheree Hughes-Stamm, PhD from Sam Houston State University describes her work on optimizing DNA extraction and STR analysis methods, and when to apply next-generation sequencing to her studies.
Integrated forensic workflow
The Applied Biosystems RapidHIT ID System generates lab-quality forensic DNA profiles in only 90 minutes with one minute of hands-on time from single-source samples. Producing STR profiles has never been this fast and easy. The compact, easy-to-use RapidHIT ID System can be set up in a day in virtually any setting, and accompanying Applied Biosystems RapidLINK Software enables real-time access, review and control.
The Applied Biosystems 3500 Series genetic analyzers now offer new features for improved data interpretation and user experience.
The Applied Biosystems SeqStudio Genetic Analyzer is a low-throughput, easy-to-use, and convenient benchtop system that delivers gold-standard CE technology with just a simple click.
The Applied Biosystems Precision ID NGS System for human identification can help you solve tough cases by getting more information from your challenging samples. By combining the Ion Chef System and either the Ion S5 or the Ion S5 XL Systems with Applied Biosystems Precision ID panels that include forensically relevant markers, adopting NGS in your laboratory is now simpler than ever.
Applied Biosystems GlobalFiler STR PCR amplification kits combine reduced amplification time with maximum data recovery power. As part of the only fully integrated and validated forensic workflow, this breakthrough 6-dye, 24-locus technology is designed to deliver superior lab performance so you can do more with less.
The Applied Biosystems Yfiler Plus PCR Amplification Kit also leverages 6-Dye technology and contains 27 Y-STR markers that are valuable to use when evidence samples are difficult to resolve with traditional autosomal STR analysis, due to low amounts of male DNA present in a high background of female DNA.
The Precision ID GlobalFiler NGS STR Panels include the same 21 autosomal STRs along with Y markers and amelogenin sex markers found in the Applied Biosystems GlobalFiler PCR Amplification Kit. In place of SE33, the Precision ID GlobalFiler NGS STR Panels contain additional multiallellic STR markers, including Penta D and Penta E in the v2 panel for the Ion S5 System, to aid in mixture interpretation of complex casework samples.
The Precision ID GlobalFiler NGS STR Panel v2 is specifically designed for sequencing on the Ion S5 and Ion S5 XL Systems. Library preparation can be performed manually, on a robotic platform, or on the Ion Chef System, with template preparation optimized for the Ion Chef System.
The Precision ID GlobalFiler NGS STR Panel is currently available for use with manual library preparation, template preparation on the Ion Chef System or the Ion OneTouch 2 System, and sequencing only on the Ion PGM System.
The Applied Biosystems Precision ID mtDNA Whole Genome Panel uses an innovative approach to mitochondrial DNA sequencing specifically developed for forensic applications. This mtDNA tiling approach includes two primer pools of 81 small amplicons in each pool, with an average size of 163 bp to assist with obtaining optimal mitochondrial genome coverage data from highly compromised, degraded samples such as hair shafts, teeth, and bones.
Precision ID mtDNA Control Region Panel uses the same mtDNA tiling approach used in the Ion AmpliSeq mtDNA Whole Genome Panel. This targeted control region panel contains two smaller sets of primer pools with 7 amplicons each that span the 1.2 kb control region, which encompasses HV-I, II, and III.
The Applied Biosystems Precision ID Ancestry Panel includes 165 autosomal markers (SNPs) that can provide you with biogeographic ancestry information and guide your investigation process.
The Applied Biosystems Precision ID Identity Panel, comprising 124 markers, provides discrimination of individuals similar to STR genotype match probabilities used by forensic analysts.
Our Human Identification Professional Services (HPS) team has been helping customers like you successfully navigate the validation process required to bring new technologies into operation. At the core of our offerings are services designed to help forensic DNA labs integrate, validate, and implement new methodologies and technologies.
Learn more from our library of Human Identification Resources.
Unless stated otherwise on the individual product labeling, products referred to are For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures. For licensing and limited use restrictions visit thermofisher.com/HIDlicensing