Optimize and automate your nucleic acid and purification workflows with Thermo Scientific KingFisher systems. KingFisher instruments reduce your hands-on time while maintaining high yields and excellent reproducibility. Optimized protocols for nucleic acids (e.g., Applied Biosystems MagMAX kits), immunoprecipitation and protein purification (e.g., Invitrogen and Applied Biosystems Dynabeads and Thermo Scientific Pierce Magnetic Beads) are provided. Thermo Scientific BindIt software allows you to modify our pre-written protocols or create your own to handle more applications.
Using KingFisher instruments with MagMAX kits provides a proven, convenient system for purifying nucleic acids for downstream applications such as real-time PCR and next-generation sequencing. Optimized MagMAX protocols can be easily downloaded from the BindIt software page.
Using KingFisher instruments with Dynabeads products gives you all of the benefits of the pioneering biomagnetic technology while increasing the number of samples you can process with less hands-on time. Dynabeads offer the best balance of high yield and reproducibility with low nonspecific binding and cost, which is one reason why they have become the gold standard for immunoprecipitation using magnetic beads. Optimized Dynabeads protocols can be easily downloaded from the BindIt software page.
Learn more about Dynabeads solutions for immunoprecipitation
Get the newest protocols for automated IP on the KingFisher platform
Using KingFisher systems with Pierce Magnetic Beads provides solid support for a variety of assays and procedures based on affinity purification. The instruments and paramagnetic particles allow users to easily mix, incubate and separate the particles in 96-well plates without columns or centrifugation. Such ease of manipulation enables rapid processing of many samples and seamless integration and compatibility with entire workflows.
The Thermo Scientific KingFisher Purification System utilizes permanent magnetic rods and disposable tip combs. When the magnetic rod—sheathed inside the tip comb—is lowered into the solution, magnetic beads collect at the bottom of the tip comb. The tip comb can then be positioned in a different row or plate, and the beads are then released by moving the magnetic rods out of the tip comb. The tip comb also facilitates the mixing of reagents with the beads by moving up and down.
| KingFisher | KingFisher mL | KingFisher Duo Prime | KingFisher Flex | KingFisher Presto (New) | |
| Max sample number/run | 24 | 15 | 12/24 per load | 96 | 96 |
|---|---|---|---|---|---|
| Volume range | 20-200 µl | 50-1000 µl | 30-5000 µl | 20-5000 µl | 50-5000 µl |
| Plate formats | strip plate | tube strips of 5 | 24DW, 96DW, elution strip | 96, 96DW, 24DW, PCR | 96, 96DW, 24DW |
| Heating | No | No | Yes | Yes | Yes |
| Cooling | No | No | Yes | No | No |
| Bar code reader | With BindIt Software | With BindIt Software | With BindIt Software; as stand alone | With BindIt Software | Part of the system |
| UV decontamination | No | No | Yes | No | No |
| Number of user selectable magnetic heads | 1 | 1 | 2 | 4 | 2 |
| Works with BindIt Software | Yes | Yes | Yes | Yes | Yes |
| Stand alone use | Yes | Yes | Yes | Yes | No* |
| Automation compatible | No | No | No | Yes | Yes, optimized |
* Protocol test run capability through BindIt Software
All KingFisher instruments come with dedicated BindIt PC software for excellent usability. Optimized protocols for a variety of Invitrogen and Thermo Scientific purification kits and reagents are included, and users can access and download new protocols for free as they become available. BindIt software also allows users to develop and optimize their own protocols, providing additional flexibility with the open KingFisher platform.
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