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Mouse IgG (H+L) Antibody (A28175) in IF
Endogenous Tubulin (green) was labeled with Mouse anti-Tubulin primary antibody (322500) (1 ug/ml) in U20S human osteocarcinoma cells and visualized using Goat anti-mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) (0.4 ug/ml, 1:2500). To demonstrate multiplexing endogenous Histone H3[AcK9] (red) was labeled with Rabbit anti-Histone H3[AcK9] primary antibody (710293) (1 ug/ml) in U20S human osteocarcinoma cells and visualized using conjugates of Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody (0.4 ug/ml, 1:2500) : (top panel) Alexa Fluor® 555 conjugate (A27039), (middle panel) Alexa Fluor® 594 conjugate (A28175) and (bottom panel) Alexa Fluor® 647 conjugate (A27040).
![Endogenous Tubulin (green) was labeled with Mouse anti-Tubulin primary antibody (322500) (1 ug/ml) in U20S human osteocarcinoma cells and visualized using Goat anti-mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) (0.4 ug/ml, 1:2500). To demonstrate multiplexing endogenous Histone H3[AcK9] (red) was labeled with Rabbit anti-Histone H3[AcK9] primary antibody (710293) (1 ug/ml) in U20S human osteocarcinoma cells and visualized using conjugates of Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody (0.4 ug/ml, 1:2500) : (top panel) Alexa Fluor® 555 conjugate (A27039), (middle panel) Alexa Fluor® 594 conjugate (A28175) and (bottom panel) Alexa Fluor® 647 conjugate (A27040).](http://tools.thermofisher.com/content/sfs/gallery/low/A28175_GAM_488_Multiplex_Tubulin20160311145026.jpg)
