Angiotensin II Induced Oxidative Stress Measured with CellROX™ Orange Reagent in Human Aortic Smooth Muscle Cells

Angiotensin –II induced oxidative stress in human smooth muscle cells (HASM) measured with CellROX™ Orange Reagent. HASM cells were plated on glass bottom 35 mm MatTek dishes. The cells were treated with or without 500 nM angiotensin-II for 4 hr at 37° C. The cells were then stained with 5 µM CellROX™ Orange Reagent and Hoechst 33342 by adding the probe to the complete media and incubating at 37° C for 30 min. The cells were washed with PBS and imaged on a Zeiss Axiovert inverted microscope using a 40x objective. Angiotensin-II treatment leads to increased oxidative stress.

Angiotensin –II induced oxidative stress in human smooth muscle cells (HASM) measured with CellROX™ Orange Reagent. HASM cells were plated on glass bottom 35 mm MatTek dishes. The cells were treated with or without 500 nM angiotensin-II for 4 hr at 37° C. The cells were then stained with 5 µM CellROX™ Orange Reagent and Hoechst 33342 by adding the probe to the complete media and incubating at 37° C for 30 min. The cells were washed with PBS and imaged on a Zeiss Axiovert inverted microscope using a 40x objective. Angiotensin-II treatment leads to increased oxidative stress.

Related Products

Related Images

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›