Compensation using the AbC™ Total Antibody Compensation Bead Kit

(A) The AbC™ Total capture beads bound to phycoerythrin (PE)-conjugated rat anti-mouse CD4 antibodies (Cat. no. MCD0404) generated positive signal in the BL2 channel (575/26) and unconjugated beads provided the negative signal. (B) The Total AbC™ capture beads bound to Alexa Fluor® 488-conjugated rat anti-mouse CD8a (Cat. no. MCD0820) generated positive signal in the BL1 channel (530/30) and unconjugated beads provided the negative signal. (C) A dual-parameter dot plot anti-mouse CD8a using compensation settings obtained with the AbC™ Total Antibody Compensation Bead Kit.

(A) The AbC™ Total capture beads bound to phycoerythrin (PE)-conjugated rat anti-mouse CD4 antibodies (Cat. no. MCD0404) generated positive signal in the BL2 channel (575/26) and unconjugated beads provided the negative signal. (B) The Total AbC™ capture beads bound to Alexa Fluor® 488-conjugated rat anti-mouse CD8a (Cat. no. MCD0820) generated positive signal in the BL1 channel (530/30) and unconjugated beads provided the negative signal. (C) A dual-parameter dot plot anti-mouse CD8a using compensation settings obtained with the AbC™ Total Antibody Compensation Bead Kit.

Related Products

Related Images

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›