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Western blot analysis of HA Epitope Tag was performed using samples from Pierce HA-Tag IP / Co-IP Kit (Product ## 26180) with SuperSignal Molecular Weight Protein Ladder (Product ## 84785) in Lane 1, an HA-tagged positive control lysate in Lane 2, unbound flow through in Lanes 3-4, and two elutions from the immobilized anti-HA resin in Lanes 5-6. The membrane was probed with an HA Epitope Tag monoclonal antibody (Product ## 26183) at a dilution of 0.1ug/ml followed by chemiluminescent detection with Thermo Scientific SuperSignal West Dura Extended Duration Substrate (Product ## 34075).
