Lipofectamine Transfection Reagent
Since launching in 1993, Invitrogen Lipofectamine reagents have become the most referenced transfection reagents with over 50,000 citations to date. You continue to choose these reagents due to their wide acceptance as the best and most reliable available. Lipofectamine reagents offer you the ability to consistently transfect many cell types with high efficiency and exceptional ease of use.
Our Lipofectamine reagent–specific protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types (see "Download protocol" links in the table below). This is often the best place to start especially in a new cell line. If you find this doesn’t work for your specific cell type, then you can you look to our cell-specific protocols for further optimization.
Which Invitrogen Lipofectamine product is right for you?
High efficiency versatile reagent for a wide range of common cell types | Most efficient versatile reagent for the widest range of cell types including difficult-to-transfect cells | Most efficient reagent for siRNA/miRNA delivery, efficient gene knock-down | Most efficient reagent for mRNA delivery. Best for neurons and primary cell types | Superior transfection efficiency in even the most difficult stem cells |
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Name | Lipofectamine 2000 Transfection Reagent |
Lipofectamine 3000 Transfection Reagent |
Lipofectamine RNAiMAX Transfection Reagent |
Lipofectamine MessengerMAX Transfection Reagent |
Lipofectamine Stem Reagent |
Top-selling format | Cat. No. 11668-019 (1.5 mL) | Cat. No. L3000-015 (1.5 mL) | Cat. No. 13778-075 (0.75 mL) | Cat. No. LMRNA008 (0.75 mL) | Cat No. STEM00008 (0.75 mL) |
Sample type |
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Transfection efficiency | High | Superior | Superior | Superior | Superior |
Cell viability | High | Superior | Superior | Superior | Superior |
Protocol | Download protocol | Download protocol | Download protocol | Download protocol | Download protocol |
Order now | Order now | Order now | Order now | Order now |
Meet the inventor
R&D transfection insights
Xavier de Mollerat du Jeu, Sr. Staff Scientist, discusses the transfection research program and key factors for successful transfections, which resulted in enhanced protocols for the Lipofectamine line of transfection reagents.
Transfection resources
More information
For Research Use Only. Not for use in diagnostic procedures.