Pacific Green dye
Pacific Green dye is a bright green-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 411/510 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.
Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm and 551 nm, respectively, facilitating three-color analysis.
We offer Pacific Green dye conjugated to a variety of primary antibodies, secondary antibodies, and streptavidin. In addition, reactive dyes and protein labeling kits are available so that you can produce your own antibody conjugates or probes.
Pacific Green dye dashboard
 Initial brightness |
Pacific Green dye is a green fluorophore used primarily for flow cytometry, optimally excited by the 405 nm violet laser line. | ||
 Photostability in buffer |
| 17 | 405 | FITC | 410 | 500 | ||
 Stain index |
 Laser line |
 Common filter set |
 Excitation max |
 Emission max |
Pacific Green dye products
We offer a range of Pacific Green dye products including labeling kits, labeled antibodies, bioconjugates, and more.
Fluorescence excitation and emission spectra of Pacific Green dye.
Pacific Green dye reactive dyes
Select dye-labeling chemistries for conjugation with the following reactive groups:
| Amine |
|---|
Violet laser–compatible antibody conjugates
Life Technologies offers Pacific Blue, Pacific Green and Pacific Orange dyes as well as Qdot probes for use with the violet laser. In addition to primary and secondary antibody conjugates of these dyes, we’ve developed kits for cell proliferation, immunophenotyping, dead cell discrimination, apoptosis, and cell cycle analysis—all aimed at helping you maximize the data you can get from your violet laser–equipped flow cytometer.
- Learn more about Cell Health and Viability Assays for Flow Cytometry
Excitation spectra (dotted lines) and emission spectra (solid lines) for Molecular Probes violet laser–excited Pacific Blue dye (blue), Pacific Green dye (green), and Pacific Orange dye (orange). The shaded area represents the emission bandpass filter (522/31) used for detection of the Pacific Green dye.
Resources
Molecular Probes Handbook
The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Flow cytometry instrument controls and standards
Keep your flow cytometry running at peak efficiency with instrument set-up, calibration, and counting beads including alignment and linearity beads, compensation tools, and cell-counting beads.
Cell stain tool
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Fluorescence SpectraViewer
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
For Research Use Only. Not for use in diagnostic procedures.