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Treatment with CultureOne Supplement improves differentiation of hPSC-derived NSCs into neurons
A. H9 ESC-derived NSCs were plated at density of 5x104 cells/cm2.
B. Without CultureOne™ Supplement, cells at 2 weeks of differentiation were highly dense, formed cell clumps, and contained MAP2-positive neurons and a significant number of SOX1-positive NSCs.
C. At 2 weeks of differentiation, cultures treated with CultureOne Supplement had an even distribution of MAP2-positive neurons with minimal SOX1-positive NSCs and no cell clumps.
D. At 5 weeks of differentiation, differentiated cells treated with CultureOne Supplement expressed mature neuronal markers neurofilament and synaptophysin. Cell nuclei were counter stained with DAPI (blue).
