For nearly 20 years, researchers have been publishing their experiments using Gibco B-27 cell culture supplements. The first commercially available, serum-free supplement for neural cell culture, Gibco B-27 Serum Free Supplement was developed for the long-term culture of rat hippocampal and cortical neurons. Since its introduction, the publication history of B-27 supplements demonstrates that researchers are expanding the use of these supplements far beyond their original applications.
B-27 Supplements
The most referenced neural cell culture supplement
Our exclusive B-27 supplements are the most referenced neural cell culture supplements. B-27 supplements have been cited in thousands of peer-reviewed publications spanning a variety of different cell types, and used in the research of many different neurological disease models.
NEW We have developed Gibco B-27 Plus Neuronal Culture System. It features an optimized formulation, upgraded manufacturing process, and more stringent quality control for raw materials and final product.
These improvements enable:
- Increased neuronal survival by more than 50%—the highest in the industry
- Accelerated neurite outgrowth
- Improved electrophysiological activity and maturation of neurons
Which B-27 supplement is right for you?
To order the right B-27 supplement for you, choose the application/cell-type tab below and then click on the product name or catalog number to order.
All Gibco neural cell culture reagents are optimized to work together as a complete media system. Need a complementary media or cells? Browse our neural cell culture product guide.
Standard Applications / Primary Neurons
| Application | Product name | Cat. No. |
|---|---|---|
| Growth and maintenance of neurons | B-27 Serum-Free Supplement | 17504044 |
| Growth and maintenance of neurons |
B-27 Plus Supplement | A3582801 |
Stem cells and Precursor cells
| Application | Product name | Cat. No. |
|---|---|---|
| Proliferation of stem cells | B-27 Supplement, minus vitamin A | 12587010 |
| Differentiation of stem cells | B-27 Supplement, XenoFree CTS™ | A14867-01 |
Specialty applications
| Application | Product name | Cat. No. |
|---|---|---|
| Studies where XenoFree components are required | B-27 Supplement, XenoFree CTS™ | A14867-01 |
| Studies of oxidative stress/damage, apoptosis, or where free radical damage to neurons occurs |
B-27 Supplement, minus AO (antioxidants) |
10889038 |
| Studies of insulin secretion or insulin receptors | B-27 Supplement, minus Insulin |
A1895601 |
| Electrophysiology studies | B-27 Electrophysiology Kit | A1413701 |
| Growth and expansion of retinal ganglion cells | B-27 Supplement, Custom | 0080085SA |
Standard Applications / Primary Neurons
| Application | Product name | Cat. No. |
|---|---|---|
| Growth and maintenance of neurons | B-27 Serum-Free Supplement | 17504044 |
| Growth and maintenance of neurons |
B-27 Plus Supplement | A3582801 |
Stem cells and Precursor cells
| Application | Product name | Cat. No. |
|---|---|---|
| Proliferation of stem cells | B-27 Supplement, minus vitamin A | 12587010 |
| Differentiation of stem cells | B-27 Supplement, XenoFree CTS™ | A14867-01 |
Specialty applications
| Application | Product name | Cat. No. |
|---|---|---|
| Studies where XenoFree components are required | B-27 Supplement, XenoFree CTS™ | A14867-01 |
| Studies of oxidative stress/damage, apoptosis, or where free radical damage to neurons occurs |
B-27 Supplement, minus AO (antioxidants) |
10889038 |
| Studies of insulin secretion or insulin receptors | B-27 Supplement, minus Insulin |
A1895601 |
| Electrophysiology studies | B-27 Electrophysiology Kit | A1413701 |
| Growth and expansion of retinal ganglion cells | B-27 Supplement, Custom | 0080085SA |
Table 1. Cell types and disease models cited in publications using Gibco neural cell culture reagents
| Primary cells | Brainstem, cerebellar, cortical, dopaminergic, DRG, enteric, GABAergic, glutamatergic, hippocampal, motor, neural progenitor, pancreatic, parasympathetic, retinal, septal, striatal, substantia nigral, sympathetic ganglia |
|---|---|
| Stem cells | Induced pluripotent stem cell, neural precursor, neural stem cell, neuroepithelial, cardiomyocytes |
| Glial cells | Astrocyte, microglia, oligodendrocytes, radial glia, satellite, schwann |
| Disease models | Alzheimer’s disease, amyotropyic lateral sclerosis (ALS), brain tumor, cerebral palsy, epilepsy, glioma, Huntington’s disease, multiple sclerosis, Parkinson’s disease, schizophrenia, spinal cord injury, stroke |
For Research Use Only. Not for use in diagnostic procedures.