Sexual Assault Casework
A key challenge: DNA mixture samples from sexual assault cases contain an excess of female DNA relative to male DNA
Male autosomal component of the DNA mixture is typically too low to be detected beyond a ratio of 1:10 to 1:20 of male:female DNA. In such cases, the use of Y-STR markers allows amplification of low quantities of male DNA in a high background of female DNA.
Generate quality results with confidence
Our 6-dye chemistries of the Applied Biosystems™ QuantiFiler™ Trio, GlobalFiler™, and Yfiler™ Plus kits provide a compatible workflow to help maximize information generated on each sample, the first time through the workflow.
Customer studies have demonstrated the ability of the QuantiFiler Trio DNA Quantification Kit to accurately predict degradation in downstream results using the GlobalFiler PCR Amplification kits. This allows laboratories to adjust input amounts to improve allele recovery. Accurate and sensitive detection of male DNA enabled by the QuantiFiler Trio allows the Yfiler Plus PCR Amplification Kit to be selected in cases of low male:female DNA ratio (M:F<1:20) where an autosomal kit would have little value.
Sexual assault workflow | Professional services | Presentations & webinars | Product listing | Resources| Video
Better decision support on STR strategy
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Highly discriminating chemistry, particularly for DNA mixture samples
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Superior Y detection using rapidly mutating Y-STRs when male:female ratio is less than 1:20
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Low male? Degraded DNA? Inhibited?
Low concentration?
Informative decision support on STR strategy using
QuantiFiler Trio ›
M:F>1:20?
Use autosomal STR such as GlobalFiler kit ›
M:F<1:20?
Select Y-STR analysis using Yfiler Plus kit ›
Look no further than our Human Identification Professional Services (HPS) team. Since 2007, the team has completed over 400 successful validation projects worldwide.
Over 20 technical support specialists, each averaging 8 years of real-world forensic experience, provide customers with in-depth training and support on our instruments, chemistries, and software.
In the first half of this webinar, Dr. Allison Holt (R&D scientist, Thermo Fisher Scientific) presents workflow improvements for analyzing challenging DNA mixture samples. During the second half, Dr. Jack Ballantyne discusses a large-scale study conducted on sexual assault samples and the ability to recover allele information using the Applied Biosystems™ QuantiFiler™ Trio and Yfiler™ Plus chemistries.
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In this webinar, Dr. Allison Holt (R&D scientist, Thermo Fisher Scientific) discusses using the Quantifiler Trio kit as decision support to streamline rape kit processing, focusing on:
- An overview of the DNA-based Y-Screen application
- Key quality features of using the Quantifiler Trio kit in casework
- Customer test results and a comparison of Y-screen versus traditional serology methods
Customer support
If you have additional comments or questions, please contact the customer service team via email.
Technical support
If you are a current customer and need technical support, please contact the global service team via email, or phone us at 1-888-821-4HID (4443). Select option #1 for immediate assistance.
For Research Forensic or Paternity Use Only. Not for use in diagnostic procedures. For licensing and limited use restrictions visit thermofisher.com/HIDlicensing