Simplify downstream protein purification with serum-free CHO Medium

  • Get optimal expression of recombinant proteins
  • Select from chemically defined, protein-free, and serum-free formulations
  • Choose media for suspension cells
  Low-protein (<100 mg/L), serum-free alternative for CHO cell line growth and production Optimizes growth of parental DHFR– CHO cells Consistent production medium High-performing, leaner medium designed for fed-batch optimization Selectively fortified for fastest fed-batch process development starting point
CHO cell line Various CHO dependent on experience DG44, DXB11, or other CHO Various CHO dependent on experience DG44, DXB11, or other CHO K1, CHO-S®, GS
Culture type** Suspension Suspension Suspension Suspension Suspension
Classification Serum-Free, contains proteins of animal origin Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined* Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined* Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined* Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined*
Relative titer in batch culture*** Medium N/A (no titer since cells are parental) High Higher Highest
Culture duration Average <14 days Average <14 days ≥14 days with feeding ≥14 days with feeding ≥20 days with feeding
Recommended Feed CHO CD EfficientFeed™ Kit CHO CD EfficientFeed™ Kit CHO CD EfficientFeed™ B CD EfficientFeed™ A CD EfficientFeed™ C
DMF reference requests Contact Technical Services Contact Technical Services Contact Technical Services Contact Technical Services Contact Technical Services
List price 1L=$87.00 1L=$87.00 1L=$84.00 1L=$76.00 1L=$93.00
Form Liquid Liquid Liquid & AGT™ Liquid & AGT™ Liquid
Liquid sizes 500mL
1L 		1L 500ml
1L
10L
20L 		1L 1L
AGT™ sizes N/A N/A 1L (24.26g)
10L (242.60g)
100L (2426.0g)
10kg		 1L (19.318g)
10L (193.180g)
100L (1931.800g)
10kg		 N/A
Option to customize Yes Yes Yes Yes Yes

* Chemically Defined means that the product does not contain any proteins or undefined components like hydrolysates or hydrolysate fractions.
** Products are intended for preparation for and use in stable cell line protein expression. See FreeStyle™ expression products for transient transfection.
*** Dependent on cell line clone