EfficientFeed™ supplements have been designed to improve CHO (Chinese hamster ovary) fed-batch culture processes for the production of recombinant proteins in suspension culture. All of these products are chemically defined and protein-free. They contain no components of animal origin.

  Best place to start if using CD OptiCHO™ base medium Best place to start if using CD CHO base medium Most concentrated feed with GS supplement
"built in" 		Fastest way to find feeding conditions that maximize CHO protein expression
Complete Feed - EfficientFeed™ A
Complete Feed - EfficientFeed™ B
Complete Feed - EfficientFeed™ C
Complete Feed - EfficientFeed™ Kit
Matching Gibco® Medium CD OptiCHO™ medium CD CHO medium CD FortiCHO™ medium CD OptiCHO™ or
CD CHO

Select if non-Gibco® medium has
<6 g/L glucose (no hydrolysates)

yes yes   yes

Select if non-Gibco® medium has
>6g/L glucose or hydrolysates

    yes  
Classification Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined* Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined* Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined* Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined*
Titer in fed-batch vs. batch 2–5 fold increase 2–5 fold increase 2.5–6 fold increase 2–5 fold increase
Culture duration >14 days in
fed-batch 		>14 days in
fed-batch 		>14 days in
fed-batch 		>14 days in
fed-batch
Volume of addition 10–50% of working volume 10–50% of working volume 10–40% of working volume 10–50% of working volume
List price 1 L = $138 1 L = $138 1 L = $147 Kit = $268
Approximate list price per liter of culture $55.00 $55.00 $44.00 $55.00
GS system supplement Add GS supplement Add GS supplement GS supplement
not needed,
already built in 		Add GS supplement
DMF
reference requests 		Contact Technical Services Contact Technical Services Contact Technical Services Contact Technical Services
Form Liquid & AGT™ Liquid & AGT™ AGT™ Liquid
Liquid sizes 1 L 1 L N/A 1 L
AGT™ sizes 10 L (651 g)
100 L (6,510 g) 		10 L (541.5 g)
100 L (5,415 g) 		1 L (79.6 g)
10 L (796 g)
100 L (7,960 g) 		N/A
Option to customize Yes Yes Yes Yes

* Chemically Defined means that the product does not contain any proteins or undefined components like hydrolysates or hydrolysate fractions