Automated Cell Counters

Compare Countess II and Countess II FL Automated Cell Counters

	Fast, easy, affordable brightfield counting Countess II Automated Cell Counter	Fluorescence counting with color options Countess II FL Automated Cell Counter
Sample results
Counting mode	Brightfield	Brightfield AND Two optional, user-changeable fluorescent channels (>20 fluorescent light cubes to choose from)
Slides	Disposable slides	Reusable slide and disposable slides
Focus	Autofocus with manual focus option
User interface	Intuitive touch screen with ability to use a mouse, if desired
Processing time	<10 seconds
	Learn more	Learn more

Advanced autofocusing and counting algorithms

The Countess Automated Cell Counter contains advanced autofocusing and counting algorithms to allow you to quickly and accurately identify and count cells within a population (Figure 1). Countess instruments are compatible with a broad range of cell types and cell lines, and we have validated their use on a number of commonly used cell lines. Upon insertion of the slide the Countess instrument will automatically focus on cells, which helps to minimize user variation associated with manual focusing. Cells between ~7 µm and 60 µm and in suspension at a density of 1 x 10⁴ to 1 x 10⁷ cells/mL are optimal for counting.

Figure 1. Autofocusing and advanced counting algorithms are designed for more precise counting. NIH/3T3, HeLa, and HL-60 cells were counted using a Countess II FL Automated Cell Counter (blue bar) or counted manually under a microscope using a hemocytometer (purple bar). Each bar is an average of 10 counts.

Clumpy cells are not a problem

Clumpy cells are not a problem for the Countess Automated Cell Counter. Whether you currently use automated or manual cell counting methods, obtaining accurate cell counts from samples containing clumpy cells can be difficult. The advanced counting algorithms of the Countess Automated Cell Counter can clearly identify cell boundaries within clumps of cells, resulting in accurate cell counts (Figure 2).