Cell Isolation With Dynabeads and MagniSort Technology
Gentle, tube-based magnetic separation with Invitrogen™ Dynabeads™ or MagniSort™ beads are technologies of choice when you want to isolate high yields of pure, viable and functional cells. Magnetic separation helps ensure that the isolated cells are not affected by passage through a dense column and that the cells’ native characteristics are preserved.
Dynabeads cell isolation products
Choose the Dynabeads cell isolation product from the categories below; also ensure that you find the right magnet for Dynabeads cell isolations
Human Cell Separation
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Mouse Cell Separation
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Separation of Other Cell Types
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Positive Isolation
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Negative Isolation
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Depletion
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Cell Separation Products
| Human Cell Separation | Mouse Cell Separation | Secondary Cell Separation |
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Positive Cell Isolation
In positive isolation, Dynabeads® bind the target cells and the bead/cell complexes are pulled to the magnet. The supernatant is discarded, leaving only your pure target cells,
| Bead-free Cells Directly after cell separation, the beads can easily be released and removed using the innovative Dynabeads® FlowComp™ technology. Other Dynabeads® release systems include the CELLection™, and the DETACHaBEAD™ technologies. |
) click image to see FlowComp™ workflow |
Negative Cell Isolation
In negative isolation, Dynabeads® are used to deplete all the unwanted cells using the supplied antibody mix. Your bead-free and untouched target cells are left in the sample, and can be directly analyzed in a flow cytometer and used in any functional assay.
- Learn More about Negative Cell Separation
Should you want to refine for a particular cell type, you can of course add extra antibodies to the antibody mix. With the secondary-coated Dynabeads® you can create your own negative isolation kit for isolation of any cells from any species.
Depletion
In depletion, cell-specific Dynabeads® are used to remove specific unwanted cell types from a mixed cell sample with >99% efficiency. Recover the depleted supernatant - still containing all your wanted cells - for downstream assays. Click for more info on: Dynabeads® for human cell depletion and mouse cell depletion.
You can also use the positively targeted and bead-bound cells for downstream molecular applications that do not require release of cells.
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Literature
MagniSort cell isolation products
Starting with T cell-specific cocktails, use the MagniSort platform to obtain enriched T cells simpler, faster and with significant cost-savings compared to traditional column-based separation methods.
Resources |
SupportNEW Cell Culture Support Center Find tips, troubleshooting help, and resources for your cell culture workflow. |
Assistance
Thermo Fisher Scientific
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