Mouse CD4+ Isolation
Which Mouse CD4+ T Cell Isolation Product is Right for You?
| Affordable & pure, flow compatible cells | Highest purity for molecular assays | High purity & physiological quality | Untouched—most physiological relevant cells | ||||
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| Isolation method | Positive isoation | Depletion or positive isolation | Positive isolation | Negative isolation | |||
| Starting samples | Any sample (whole blood, spleen, lymph node) | Any sample (whole blood, spleen, lymph node) | Any sample (whole blood, spleen, lymph node) | Spleen, lymph node cells* | |||
| # Cells processed | 2 x 109 cells | 1-2 x 109 cells | 2 x 109 cells | 1 x 109 cells | |||
| Purity | Higher
90-98% | Higher
90-98% | Higher
90-98% | High
80-90% |
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| Yield | Standard
50-60% | Highest
95-100% | Standard
50-70% | Standard
50-70% |
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| Viability | >90% | >90% | >90% | >90% | |||
| Isolation time | ~60min | ~30 min | ~90 min | ~50 min | |||
| Isolation temperature | cold | cold | release at room temperature | cold | |||
| Applications | Cellular assays
(cell culture, cell activation/proliferation, cytokine studies) Molecular: isolation of proteins & nucleic acids | Depletion of unwanted CD4+ T cells or
Positive isolation for molecular isolation of proteins & nucleic acids | Cellular assays
(cell culture, cell activation/proliferation, cytokine studies) Translational research Molecular: isolation of proteins & nucleic acids | Cellular applications for physiological studies (functional studies, functional studies, adoptive transfer, flow analysis or further sorting) | |||
| Bead-free target cells | Yes
(but antibody present) | No | Yes
(and antibody-free) | Yes | |||
| Compatible with
flow cytometry /flow sorting | ![]() | ![]() | ![]() |
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| Shelf life from production date | 2 years | 3 years | 3 years | 3 years | |||
| Required materials | Magnets | Magnets | Magnets | Magnets | |||
| Order Now | Order Now | Order Now | Order Now |
THIS IS FOR MOUSE RESEARCH - NOT FOR HUMAN

