Which Mouse T Cell Isolation Product is Right for You?
| Affordable & pure, flow compatible cells | Highest purity for molecular assays | Untouched—most physiological relevant cells | |||
|---|---|---|---|---|---|
|
|
|
|
|||
| Isolation method | Positive isolation | Depletion or positive isolation | Negative isolation | ||
| Starting samples | Any sample (whole blood, spleen, lymph node) | Any sample (whole blood, spleen, lymph node) | Spleen, lymph node cells* | ||
| # cells processed | 2 x 109 cells | 1–2 x 109 cellls | 1 x 109 cells | ||
| Purity | Higher >90% | Higher >90% | High >85% | ||
| Yield | Standard >55% | Highest >95% | High >70% | ||
| Viability | >90% | >90% | >90% | ||
| Isolation time | ~60min | ~30 min | ~50 min | ||
| Isolation temperature | cold | cold | cold | ||
| Applications | Cellular assays (cell culture, cell activation/proliferation, cytokine studies) Molecular: isolation of proteins & nucleic acids | Depletion of unwanted T cells or Positive isolation for molecular: isolation of proteins & nucleic acids | Cellular applications for physiological studies (functional studies, adoptive transfer, flow analysis or further sorting) | ||
| Bead-free target cells | Yes (but antibody present) | No | No | ||
| Compatible with flow cytometry /flow sorting | Yes | No | Yes | ||
| Shelf life from production date | 2 years | 3 years | 3 years | ||
| Required materials | Magnets | Magnets | Magnets | ||
| Order Now | Order Now | Order Now |
* Lyse the red cells prior to use to improve the purity
THIS IS FOR MOUSE RESEARCH - NOT FOR HUMAN
