First-generation Attune flow cytometer support

Find technical resources for the original first-generation Invitrogen™ Attune™ Flow Cytometer, including manuals, reagent selection tables, software download information, and ordering information for consumables and accessories for your instrument.

Expand your research capabilities with the next-generation Attune NxT Flow Cytometer

Reagent selection guide for the original Attune Flow Cytometer

Blue/violet instrument configuration
Excitation laser Default filter (nm) Filter range (nm) Fluorophores Other fluorescent reagents
Violet
405 nm		 450/40 430–470 Alexa Fluor 405
Pacific Blue		 PO-PRO-1
DyeCycle Violet
Fixable Violet Dead Cell Stain
CellTrace Violet
Calcein Violet
SYTOX Blue
FxCycle Violet
Click-iT Pacific Blue
522/31 507–537 Qdot 525
Horizon V500		 Fixable Aqua Dead Cell Stain
F2N12S (apoptotic)
603/48 579–627 Pacific Orange
Qdot 605		 Fixable Yellow Dead Cell Stain
F2N12S (live)
Blue
488 nm		 530/30 515–545 Alexa Fluor 488
Fluorescein		 Calcein
Fluo-3/Fluo-4
TO-PRO-1 iodide
CFSE
GFP
JC-1/DiOC2(3)
SYTOX Green
DyeCycle Green
Rhodamine 123
YO-PRO-1 iodide
Fixable Green Dead Cell Stain
Click-iT Alexa Fluor 488
574/26 561–587 PE
 PI
Fura Red AM cell permeant
DyeCycle Orange
JC-1/DiOC2(3)
pHrodo Phagocytosis Particle Labeling Kit
SNARF (low pH) isomers
SYTOX Orange Dead cell stain
640LP >640 PE-Alexa Fluor 610
PerCP
PE-Alexa Fluor 700
PE-Cy® 5.5
TRI-COLOR (TC)
PE-Cy®5
PerCP-Cy® 5.5
PE-Cy® 7
PE-PE-Alexa Fluor 750
Qdot 655
Qdot 705
Qdot 800		 JC-1/DiOC2(3)
Fixable Red Dead Cell Stain
7-AAD
SNARF (high pH) isomers
SYTOX AADvanced Dead Cell Stain
DyeCycle™ Ruby
Blue/red instrument configuration
Excitation laser Default filter (nm) Filter range (nm) Fluorophores Other fluorescent reagents
Blue
488 nm		 530/30 515–545 Alexa Fluor 488
Fluorescein		 Calcein
Fluo-3/Fluo-4
TO-PRO-1 iodide
CFSE
GFP
JC-1/DiOC2(3)
SYTOX Green
DyeCycle™ Green
Rhodamine 123
YO-PRO-1 iodide
Fixable Green Dead Cell Stain
Click-iT Alexa Fluor 488
574/26 561–587 PE
 PI
Fura Red™ AM cell permeant
DyeCycle™ Orange
JC-1/DiOC2(3)
pHrodo™ Phagocytosis Particle Labeling Kit
SNARF (low pH) isomers
SYTOX Orange Dead cell stain
690/50 665–715 Qdot 705
PerCP
TRI-COLOR
PE-Cy®5
PerCP-Cy®5.5
PE-Cy®5.5		 Vybrant Dye Cycle Ruby
780/60 750–810 PE-Alexa Fluor 750
Qdot 800
PE-Cy®7		 Vybrant Dye Cycle Ruby
Red
637 nm		 660/20 650–670 Allophycocyanin (APC)
Alexa Fluor 647		 Fixable Far Red Dead Cell Stain
Click-iT Alexa Fluor 647
FxCycle Far Red
SYTOX Red Dead cell stain
TO-PRO-3
780/60 750–810 APC-Alexa Fluor 750
APC-Cy®7		 Fixable Near-IR Dead Cell Stain
Vybrant Dye Cycle Ruby

Attune Autosampler

The Invitrogen™ Attune™ NxT Autosampler is compatible with the original Attune flow cytometer and gives you the flexibility of automated multi-sample processing, offering:

  • Broad compatibility—compatible with many different plate formats, including 96-well, 384-well, and deep-well plates.
  • Mixing by aspiration—mixing sample by aspiration instead of shaking ensures homogeneity of the sample and helps maintains cell viability
  • Intelligent probe—intelligent probe design minimizes clogging and carryover (<0.5%) and prevents damage to the instrument
  • Auto cleaning—performs automated cleaning when the instrument is shutting down
  • Consistent data—Designed to provide minimal variation regardless of sampling method (tube vs. plate) and collection rate

See how the Autosampler works with the Attune NxT flow cytometer by requesting an in-lab demo from one of our trained representatives.

Request a demo  Request a quote

Attune cytometric software

The software for the original Attune Flow Cytometer can be downloaded without licensing fees and can be added to any desktop or laptop computer at your institution without any additional costs. Results can be easily analyzed at your convenience at your own computer, allowing the next user to run experiments on the Attune cytometer. Before initiating the download process, use the table below to determine which software version your system uses.

  Attune Cytometer
WITHOUT Autosampler		 Attune Cytometer
WITH Autosampler
  photo of the original Attune without Autosampler Original Attune without Autosampler
Purchase date of Attune Cytometer BEFORE
January 1st, 2013		 AFTER
January 1st, 2013

—
Software version v1.2.5 * v2.1.0 † v2.1.0 †
Operating system Windows™ XP 64-bit Windows 7 64-bit Windows 7
* To get the benefits of the v2.1.0 software requires the purchase of an Upgrade Kit; or you can upgrade to v2.1.0 for free with the purchase of an Attune Autosampler.  † Version 2.1.0 requires a 64-bit Windows 7 computer. 8 GB RAM with minimal 500 GB storage are recommended.

Why should I upgrade to v2.1.0?

If you purchased your Attune Cytometer without an Autosampler before January 1, 2013, you may be running software v1.2.5 on a Windows XP system. To get the benefits of the v2.1.0 software requires the purchase of an upgrade kit; or you can upgrade to v2.1.0 for free with the purchase of an Attune Autosampler.

  • Better performance—responsiveness of software v2.1.0 is dramatically improved over v1.2.5
  • Faster analysis of samples—save time with batch processing, ability to select multiple objects, and easier experiment setup and data analysis
  • Sample recovery—no other syringe-driven system offers the ability to recover unused samples
  • Increased functionality—additional functionality beyond supporting the use of 96- and 384-well plates

The Attune Computer Upgrade Kit (Cat. No. 4481653) includes:

  • A Dell™ 64-bit computer with the Windows 7 operating system
  • Attune Cytometric Software v2.1 and v2.1 Documentation Kit
  • A visit by trained service personnel to assist in the transfer of data from the existing computer system

Download the Upgrade Kit Flyer

There are two ways to purchase the upgrade kit:

  1. Standalone kit: Attune Software (v2.1.0) and Computer Upgrade Kit, including on-site installation and data transfer (Cat. No. 4481653); or
  2. Kit + Service Contract: 1-year service contract and the upgrade kit together (35% savings compared to purchasing separately). Contact your sales rep to request a quote.

Resources

For Research Use Only. Not for use in diagnostic procedures.