Click-iT® Assay Kits   Click-iT® technology provides a streamlined and simplified workflow for multiple applications in microscope imaging, flow cytometry, and high-content analysis or screening. We’ve developed a series of optimized assay kits designed to generate reliable results, including kits to measure cell proliferation, new protein synthesis, RNA synthesis, lipid peroxidation, and apoptosis using TUNEL assays. Additionally, Click-iT® technology can be used as a sensitive tool for molecular capture, and we offer kits to capture nascent proteins and RNA. The topics below will assist you in selecting the right kit based on your assay platform and application.

Click-iT® Assay Kits

Assay kits for imaging

Click-iT® Assay Kits for imaging provide a robust workflow with a selection of detection options to allow for multiplexing and provide a broader biological context for your results. Imaging kits are optimized for microscopy and offered with a range of Alexa Fluor® dyes for detection.

Assay kits for HCS

Click-iT® HCS Assay kits are optimized for higher throughput and provide reagent for multiple microplates in each kit. Not all imaging kits are provided in a high throughput format.

  
Click-iT® Plus EdU Alexa Fluor® 488 Imaging KitProliferating HeLa cells detected using the Click-iT® Plus EdU Alexa Fluor® 488 Imaging Kit.

Assay kits for flow cytometry

Click-iT® Assay Kits for Flow Cytometry provide the most convenient to measure cell proliferation by tracking new DNA synthesis. Cell proliferation assays are offered with a range of Alexa Fluor® dyes for detection to allow for multiplexing with common fluorophores, including R-PE and R-PE tandems, as well as fluorescent proteins.

Kits for biomolecule capture

When Click-iT® technology is used for molecular capture, it enables the enrichment of newly synthesized RNA or protein species that can be used in subsequent gene expression analysis or protein sequencing.

   Click-iT® Plus EdU Alexa Fluor® 488 Kit

Compatibility of Click-iT® Plus EdU reaction with R-PE tandem conjugate (R-PE Cy®7) fluorescence.

Ordering information

Product Quantity Cat. No.
Click-iT® kits for protein analysis
Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit 1 kit C10428
Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit 1 kit C10429
Click-iT® Plus OPP Alexa Fluor® 488 Protein Synthesis Assay Kit 1 kit C10456
Click-iT® Plus OPP Alexa Fluor® 594 Protein Synthesis Assay Kit 1 kit C10457
Click-iT® Plus OPP Alexa Fluor® 647 Protein Synthesis Assay Kit 1 kit C10458
Click-iT® kits for cell proliferation
Click-iT® EdU Alexa Fluor® 488 Imaging Kit 1 kit, 50 to 250 coverslips C10337
Click-iT® EdU Alexa Fluor® 555 Imaging Kit 1 kit, 50 to 250 coverslips C10338
Click-iT® EdU Alexa Fluor® 594 Imaging Kit 1 kit, 50 to 250 coverslips C10339
Click-iT® EdU Alexa Fluor® 647 Imaging Kit 1 kit, 50 to 250 coverslips C10340
Click-iT® Plus EdU Alexa Fluor® 488 Imaging Kit 1 kit, 50 to 250 coverslips C10637
Click-iT® Plus EdU Alexa Fluor® 555 Imaging Kit 1 kit, 50 to 250 coverslips C10638
Click-iT® Plus EdU Alexa Fluor® 594 Imaging Kit 1 kit, 50 to 250 coverslips C10639
Click-iT® Plus EdU Alexa Fluor® 647 Imaging Kit 1 kit, 50 to 250 coverslips C10640
Click-iT® kits for TUNEL assays
Click-iT® TUNEL Alexa Fluor® 488 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10245
Click-iT® TUNEL Alexa Fluor® 594 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10246
Click-iT® TUNEL Alexa Fluor® 647 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10247
Click-iT® kits for RNA synthesis assays
Click-iT® RNA Alexa Fluor® 488 Imaging Kit 1 kit C10329
Click-iT® RNA Alexa Fluor® 594 Imaging Kit 1 kit C10330
Click-iT® kits for lipid peroxidation assays
Click-iT® Lipid Peroxidation Imaging Kit, Alexa Fluor® 488 1 kit, 500 assays C10446
Product Quantity Cat. No.
Click-iT® kits for nascent protein capture
Click-iT® Protein Enrichment Kit, for click chemistry capture of azide-modified proteins 1 kit C10416
Click-iT® kits for nascent RNA capture
Click-iT® Nascent RNA Capture Kit, for gene expression analysis 1 kit C10365
Assay kits for imaging
Product Quantity Cat. No.
Click-iT® kits for protein analysis
Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit 1 kit C10428
Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit 1 kit C10429
Click-iT® Plus OPP Alexa Fluor® 488 Protein Synthesis Assay Kit 1 kit C10456
Click-iT® Plus OPP Alexa Fluor® 594 Protein Synthesis Assay Kit 1 kit C10457
Click-iT® Plus OPP Alexa Fluor® 647 Protein Synthesis Assay Kit 1 kit C10458
Click-iT® kits for cell proliferation
Click-iT® EdU Alexa Fluor® 488 Imaging Kit 1 kit, 50 to 250 coverslips C10337
Click-iT® EdU Alexa Fluor® 555 Imaging Kit 1 kit, 50 to 250 coverslips C10338
Click-iT® EdU Alexa Fluor® 594 Imaging Kit 1 kit, 50 to 250 coverslips C10339
Click-iT® EdU Alexa Fluor® 647 Imaging Kit 1 kit, 50 to 250 coverslips C10340
Click-iT® Plus EdU Alexa Fluor® 488 Imaging Kit 1 kit, 50 to 250 coverslips C10637
Click-iT® Plus EdU Alexa Fluor® 555 Imaging Kit 1 kit, 50 to 250 coverslips C10638
Click-iT® Plus EdU Alexa Fluor® 594 Imaging Kit 1 kit, 50 to 250 coverslips C10639
Click-iT® Plus EdU Alexa Fluor® 647 Imaging Kit 1 kit, 50 to 250 coverslips C10640
Click-iT® kits for TUNEL assays
Click-iT® TUNEL Alexa Fluor® 488 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10245
Click-iT® TUNEL Alexa Fluor® 594 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10246
Click-iT® TUNEL Alexa Fluor® 647 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10247
Click-iT® kits for RNA synthesis assays
Click-iT® RNA Alexa Fluor® 488 Imaging Kit 1 kit C10329
Click-iT® RNA Alexa Fluor® 594 Imaging Kit 1 kit C10330
Click-iT® kits for lipid peroxidation assays
Click-iT® Lipid Peroxidation Imaging Kit, Alexa Fluor® 488 1 kit, 500 assays C10446
Assay kits for HCS
Product Quantity Cat. No.
Click-iT® kits for protein analysis
Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay 1 kit, 2 plates C10289
Click-iT® kits for cell proliferation
Click-iT® EdU Alexa Fluor® 488 HCS Assay 1 kit, 2 plates C10350
Click-iT® EdU Alexa Fluor® 488 HCS Assay 1 kit, 10 plates C10351
Click-iT® EdU Alexa Fluor® 555 HCS Assay 1 kit, 2 plates C10352
Click-iT® EdU Alexa Fluor® 555 HCS Assay 1 kit, 10 plates C10353
Click-iT® EdU Alexa Fluor® 594 HCS Assay 1 kit, 2 plates C10354
Click-iT® EdU Alexa Fluor® 594 HCS Assay 1 kit, 10 plates C10355
Click-iT® EdU Alexa Fluor® 647 HCS Assay 1 kit, 2 plates C10356
Click-iT® EdU Alexa Fluor® 647 HCS Assay 1 kit, 10 plates C10357
Click-iT® EdU Microplate Assay 1 kit, 4 plates C10214
Click-iT® kits for TUNEL assays
Click-iT® TUNEL Alexa Fluor® 488 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10245
Click-iT® TUNEL Alexa Fluor® 594 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10246
Click-iT® TUNEL Alexa Fluor® 647 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10247
Click-iT® kits for RNA synthesis assays
Click-iT® RNA Alexa Fluor® 488 HCS Assay 1 kit C10327
Click-iT® RNA Alexa Fluor® 594 HCS Assay 1 kit C10328
Click-iT® kits for lipid peroxidation assays
Click-iT® Lipid Peroxidation Imaging Kit, Alexa Fluor® 488 1 kit, 500 assays C10446
Assay kits for flow cytometry
Product Quantity Cat. No.
Click-iT® assay kits for cell proliferation
Click-iT® EdU Pacific Blue™ Flow Cytometry Assay Kit 1 kit, 50 tests C10418
Click-iT® EdU Alexa Fluor® 488 Flow Cytometry Assay Kit 1 kit, 50 tests C10425
Click-iT® EdU Alexa Fluor® 488 Flow Cytometry Assay Kit 1 kit, 100 tests C10420
Click-iT® EdU Alexa Fluor® 647 Flow Cytometry Assay Kit 1 kit, 50 tests C10424
Click-iT® EdU Alexa Fluor® 647 Flow Cytometry Assay Kit 1 kit, 100 tests C10419
Click-iT® Plus EdU Alexa Fluor® 488 Flow Cytometry Assay Kit 1 kit, 50 tests C10632
Click-iT® Plus EdU Alexa Fluor® 488 Flow Cytometry Assay Kit 1 kit, 100 tests C10633
Click-iT® Plus EdU Alexa Fluor® 647 Flow Cytometry Assay Kit 1 kit, 50 tests C10634
Click-iT® Plus EdU Alexa Fluor® 647 Flow Cytometry Assay Kit 1 kit, 100 tests C10635
Kits for biomolecule capture
Product Quantity Cat. No.
Click-iT® kits for nascent protein capture
Click-iT® Protein Enrichment Kit, for click chemistry capture of azide-modified proteins 1 kit C10416
Click-iT® kits for nascent RNA capture
Click-iT® Nascent RNA Capture Kit, for gene expression analysis 1 kit C10365

Resources

Learn more

Molecular Probes® Handbook

  

BioProbes® Journal articles

Scientific poster