RNA Extraction From Cultured Cells
Purifying RNA from cultured cells has traditionally been a highly repetitive, time- and labor-intensive procedure, often involving the use of toxic organic chemicals. The products developed by our RNA experts allow you to isolate RNA of maximum yield, purity, and integrity using several technology formats (organic extraction, glass-fiber columns, magnetic beads, and combinations of these).
You can also analyze cultured cell lysates without first isolating the RNA. In significantly less time, you’ll get qPCR results equivalent to - or in some cases better than - those from purified RNA. The Cells-to-CT™ and Single Cell-to-CT™ product lines offer tremendous performance and handling advantages.
Which RNA Isolation Kit from Cultured Cells is Right for You?
| Highly pure, intact RNA | Choice for long template with enhanced fidelity & specificity | Complete system—cells to qRT-PCR with TaqMan® Master Mix | Complete system—cells to qRT-PCR from 1-10 cells | |
|---|---|---|---|---|
| Order now | Order now | Order now | Order now | |
| TRIzol® Reagent | Power SYBR® Green Cells-to-CT™ Kit | TaqMan® Gene Expression Cells-to-CT™ Kit | Ambion® Single Cell-to-CT™ Kit | |
| Recommendations | Most Cited, Top Seller | Top Seller | Top Innovation | |
| Prep time | 1 hr | <10 minutes | <10 minutes | <10 minutes |
| Cell lysis method | High purity organic extraction (requires alcohol precipitation) | Cells-to-CT™ lysis | Cells-to-CT™ lysis | Cells-to-CT™ lysis |
| Pre-amplification reagents included | No | No | No | TaqMan® PreAmp Master Mix |
| Reverse transcription reagents included | No | MuLV | MuLV | Superscript VILO™ |
| PCR Enzyme included | No | AmpliTaq Gold® | AmpliTaq Gold® | Platinum® Taq Hi-Fi |
| Master mix included | No | SYBR® Green | TaqMan® | TaqMan® |
| Amount of Starting material | Up to 1 x 107 cells | 10–100,000 cells | 10–100,000 cells | 1–10 cells |
| Application | All RNA expression methods | qRT-PCR | qRT-PCR | qRT-PCR |
Preserve and Stabilize Your RNA
RNAlater® Solution is widely used for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It eliminates the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.