RNA Isolation from Blood
To avoid sample breakdown and allow for the most accurate analysis and profiling results, a stabilization reagent should be added to the blood samples prior to RNA purification. This also allows for long term storage of samples.
To minimize the high ribonuclease (RNase) activity in blood plasma, we offer two options: 1) blood samples can be drawn directly into Tempus™ Blood RNA Tubes, which contain a stabilizing reagent, 2) addition of RNAlater® Stabilization Solution immediately after blood sample collection.”
Which RNA extraction kit from blood sample is right for you?
| Order now | Order now | Order now | Order now | Order now | |
| Optimized for liquid samples | Specialized kit deplete non-nucleated erythrocytes | HTP optimized for purification from stabilized blood samples | HTP format,capable of recovering RNA of all sizes (including microRNAs) | High RNA yield directly from whole blood, no need to isolate WBCs | |
|---|---|---|---|---|---|
| TRIzol® LS | PureLink™ Total RNA Blood Kit | MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit | MagMAX mirVana Total RNA Isolation | RiboPure™ Blood Kit | |
| Top Seller | ✓ | ||||
| Compatible stabilization reagents | EDTA, Heparin, Citrate, RNAlater® | EDTA, Heparin, Citrate | Tempus™ or PaxGene® tubes | EDTA or Citrate | EDTA, Heparin, Citrate, RNAlater® |
| Stabilization reagent included | ✓ RNAlater® |
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| Isolation method | High purity organic extraction (requires alcohol precipitation) | Fast, convenient silica column | Scalable, flexible format with magnetic beads | Scalable, flexible format with magnetic beads | Highest purity & convenience; includes both organic extraction & silica column (no precipitation required) |
| Prep time | 1 hour | 20 minutes | 12 tubes in 2 hours | 96 samples in <1 hr | 30 minutes |
| High throughput compatible | ✓ | ✓ |
For more info on working with blood please review our technical articles:
TechNotes Articles
- Tips & Tricks for Working with Blood Samples
- Extract MicroRNA from Blood Samples - TechNotes 13(1)
- High Throughput RNA Recovery from Mammalian, Plant, and Viral Samples - TechNotes 13(1)
- Improve RNA Isolation for Array Analysis and More - TechNotes 10(3)
- Improved Gene Expression Profiling With Mouse Blood Samples - TechNotes 13(4)
- Improved Methods for Gene Expression Profiling from Blood Samples - TechNotes 13(3)
- Improved Microarray Sensitivity using Whole Blood RNA Samples - TechNotes 12(3)
- Isolate RNA from White Blood Cells Captured by a Novel Filter System - TechNotes 12(4)
- Low and High Throughput RNA Isolation from Tissues, Cell-free Samples, and Blood - TechNotes 13(2)
- miRNA Expression in White Blood Cells - TechNotes 11(3)
- Obtaining the Highest Quality RNA from Blood Samples - TechNotes 16(1)
- Purify RNA Directly from Whole Blood - TechNotes 11(4)
- Quantitate miRNA from a Few Drops of Mouse Blood - TechNotes 14(4)
- Research Highlight: Superior Microarray Data from Blood Samples - TechNotes 14(4)
- RNA Extraction from Blood: Choosing the Best System for Your Gene Expression Studies - TechNotes 14(2)
- RNA from Mouse Whole Blood for Expression Profiling - TechNotes 13(3)
- Robust High Throughput RNA Isolation from Blood Samples - TechNotes 12(3)
- Superior Gene Expression Profiling with Human Whole Blood Samples - TechNotes 14(3)
- Total RNA from Whole Blood for Expression Profiling - TechNotes 12(2)
